ArticleACS chemical biology2012
Regulation of transcription through light-activation and light-deactivation of triplex-forming oligonucleotides in mammalian cells.
Article in ACS chemical biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 68 citations in OpenAlex.
- The Development of Non-natural Type Nucleoside to Stabilize Triplex DNA Formation against CG and TA Inversion Site.Current medicinal chemistry · 2024Review
- Expanding Catch and Release DNA Decoy (CRDD) Technology with Pyrimidine Mimics.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Optical Control of MicroRNA Function in Zebrafish Embryos.Journal of the American Chemical Society · 2022Article
- Site-specific photolabile roadblocks for the study of transcription elongation in biologically complex systems.Communications biology · 2022Article
- Selective and stable base pairing by alkynylated nucleosides featuring a spatially-separated recognition interface.Nucleic acids research · 2022Article
- Deactivation of a dimeric DNA-binding peptide through a palladium-mediated self-immolative cleavage.RSC advances · 2022Article
- Translational control of gene function through optically regulated nucleic acids.Chemical Society reviews · 2021Review
- LED control of gene expression in a nanobiosystem composed of metallic nanoparticles and a genetically modified E. coli strain.Journal of nanobiotechnology · 2021Article
- Light-induced modulation of DNA recognition by the Rad4/XPC damage sensor protein.RSC chemical biology · 2021Article
- Regulating CRISPR/Cas9 Function through Conditional Guide RNA Control.Chembiochem : a European journal of chemical biology · 2021Review
- Controlling gene expression with light: a multidisciplinary endeavour.Biochemical Society transactions · 2020Review
- Modification of the aminopyridine unit of 2'-deoxyaminopyridinyl-pseudocytidine allowing triplex formation at CG interruptions in homopurine sequences.Nucleic acids research · 2018Article
- Optochemical Control of Biological Processes in Cells and Animals.Angewandte Chemie (International ed. in English) · 2018Review
- Caged circular siRNAs for photomodulation of gene expression in cells and mice.Chemical science · 2018Article
- Stimuli-responsive oligonucleotides in prodrug-based approaches for gene silencing.Beilstein journal of organic chemistry · 2018Review
- Optically Triggered Immune Response through Photocaged Oligonucleotides.Tetrahedron letters · 2015Article
- Site-specific promoter caging enables optochemical gene activation in cells and animals.Journal of the American Chemical Society · 2014Article
- Optochemical control of deoxyoligonucleotide function via a nucleobase-caging approach.Accounts of chemical research · 2014Article
- Optochemical control of RNA interference in mammalian cells.Nucleic acids research · 2013Article
- Cellular delivery and photochemical activation of antisense agents through a nucleobase caging strategy.ACS chemical biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Triplex-forming oligonucleotides (TFOs) are efficient tools to regulate gene expression through the inhibition of transcription. Here, nucleobase-caging technology was applied to the temporal regulation of transcription through light-activated TFOs. Through site-specific incorporation of caged thymidine nucleotides, the TFO:DNA triplex formation is blocked, rendering the TFO inactive. However, after a brief UV irradiation, the caging groups are removed, activating the TFO and leading to the inhibition of transcription. Furthermore, the synthesis and site-specific incorporation of caged deoxycytidine nucleotides within TFO inhibitor sequences was developed, allowing for the light-deactivation of TFO function and thus photochemical activation of gene expression. After UV-induced removal of the caging groups, the TFO forms a DNA dumbbell structure, rendering it inactive, releasing it from the DNA, and activating transcription. These are the first examples of light-regulated TFOs and their application in the photochemical activation and deactivation of gene expression. In addition, hairpin loop structures were found to significantly increase the efficacy of phosphodiester DNA-based TFOs in tissue culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.