ArticleNorth American journal of medical sciences2011
Direct molecular detection of Mycobacterium tuberculosis complex from clinical samples - An adjunct to cultural method of laboratory diagnosis of tuberculosis.
Article in North American journal of medical sciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Human and camel cystic echinococcosis - a polyclonal antibody-based sandwich ELISA for its serodiagnosis with molecular identification.Veterinary research communications · 2024Article
- First detection of bovine tuberculosis by Ziehl-Neelsen staining and polymerase chain reaction at dairy farms in the Lekok Sub-District, Pasuruan Regency, and Surabaya region, Indonesia.Veterinary world · 2024Article
- Biosensors; nanomaterial-based methods in diagnosing ofJournal of clinical tuberculosis and other mycobacterial diseases · 2024Review
- Article
- The development and use of ActiphageMicrobial biotechnology · 2020Article
- Identification of Candidate Genes Associated with Charcot-Marie-Tooth Disease by Network and Pathway Analysis.BioMed research international · 2020Article
- Identification of spore-forming bacteria isolated from contaminated Lowenstein Jensen media and effectiveness of Vancomycin to reduce Mycobacterial culture contamination in Burkina-Faso.Scientific reports · 2019Article
- Pathway-based network analyses and candidate genes associated with Kashin-Beck disease.Medicine · 2019Review
- Identification ofFrontiers in cellular and infection microbiology · 2017Article
- Drug resistant strains of Mycobacterium tuberculosis identified through PCR-RFLP from patients of Central Punjab, Pakistan.International journal of immunopathology and pharmacology · 2016Article
- Chlorhexidine decontamination of sputum for culturing Mycobacterium tuberculosis.BMC microbiology · 2015Article
- Mycobacterium Tuberculosis Infection within a Warthin Tumor: A Case Report and Literature Review.North American journal of medical sciences · 2013Article
- Pott's Spine: Diagnostic Imaging Modalities and Technology Advancements.North American journal of medical sciences · 2013Review
- Menstrual Blood versus Endometrial Biopsy in Detection of Genital Tuberculosis by Using Nested Polymerase Chain Reaction in an Endemic Region.Journal of human reproductive sciencesArticle
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundTuberculosis, a communicable disease with significant morbidity and mortality, is the leading cause of death in the world from bacterial infectious disease. Because of its public health importance, there is need for rapid and definitive method of detecting the causative organism. Several approaches have been attempted, but the molecular methods, especially Polymerase Chain Reaction assays are the most promising for rapid detection of Mycobacterium tuberculosis complex from clinical samples.
aimThis study was aimed at using Polymerase Chain Reaction for detection of Mycobacterium tuberculosis complex from clinical samples using universal sample processing methodology. SUBJECTS AND
methodsTwo hundred clinical samples sent to Tuberculosis laboratories in Ibadan and Osogbo, Nigeria, were enrolled in this study. The samples were processed by universal sample processing methodology for PCR; smear microscopy was carried out on sputum samples by Ziehl Nelseen staining technique; and cultured on Middlebrook agar medium containing oleic acid albumin dextrose complex supplement after decontamination of samples.
resultsNinety six (48%) samples were detected positive for M. tuberculosis complex by polymerase chain reaction using the combination of boiling and vortexing and microscopy detected 72 (36%) samples positive for acid fast bacilli. Using culture method as gold standard, it was found that polymerase chain reaction assay was more sensitive (75.5%) and specific (94.8%) than microscopy (sensitivity of 48.5% and specificity of 85.7%) in detecting M. tuberculosis complex from clinical samples. There was significant difference in detecting M. tuberculosis from clinical samples when compared to microscopy (p<0.05).
conclusionThe study recommends that direct molecular detection of M. tuberculosis complex is sensitive and specific and polymerase chain reaction method should be used as an adjunct to other methods of laboratory diagnosis of tuberculosis.
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