ArticlePloS one2012
Survival or revival: long-term preservation induces a reversible viable but non-culturable state in methane-oxidizing bacteria.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Metabolomics in Microbial Genetic Resource Conservation: From Strain Identity to Functional Preservation.Methods in molecular biology (Clifton, N.J.) · 2027Review
- How Salmonella Works Under Osmotic and Desiccation Stresses.Comprehensive reviews in food science and food safety · 2026Review
- Polyphasic Characterization ofIndian journal of microbiology · 2025Article
- Candida species-specific colonization in the healthy and impaired human gastrointestinal tract as simulated using the Mucosal Ileum-SHIME® model.FEMS microbiology ecology · 2024Article
- Study on methane degradation by microbial agents based on chelating wetting agent carriers.Scientific reports · 2024Article
- The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria.Microorganisms · 2023Review
- Transcriptomic responses of the fast-growing bacterium Vibrio natriegens during cold-induced loss of culturability.Applied microbiology and biotechnology · 2023Article
- The Next Generation of Microbial Ecology and Its Importance in Environmental Sustainability.Microbial ecology · 2023Review
- Identification of a TonB-Dependent Receptor Involved in Lanthanide Switch by the Characterization of Laboratory-Adapted Methylosinus trichosporium OB3b.Applied and environmental microbiology · 2023Article
- Overview of VBNC, a survival strategy for microorganisms.3 Biotech · 2022Review
- Effect of cryopreservation medium conditions on growth and isolation of gut anaerobes from human faecal samples.Microbiome · 2022Article
- Development of a reproducible small intestinal microbiota model and its integration into the SHIMEFrontiers in microbiology · 2022Article
- Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms.Marine life science & technology · 2021Review
- Comparison of stress conditions to induce viable but non-cultivable state in Salmonella.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Evaluation of growth and sporulation of a non-toxigenic strain of Clostridioides difficile (Z31) and its shelf viability.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Improving End-User Trust in the Quality of Commercial Probiotic Products.Frontiers in microbiology · 2019Review
- Article
- The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage.Microbial cell factories · 2014Article
- Optimized cryopreservation of mixed microbial communities for conserved functionality and diversity.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knowledge on long-term preservation of micro-organisms is limited and research in the field is scarce despite its importance for microbial biodiversity and biotechnological innovation. Preservation of fastidious organisms such as methane-oxidizing bacteria (MOB) has proven difficult. Most MOB do not survive lyophilization and only some can be cryopreserved successfully for short periods. A large-scale study was designed for a diverse set of MOB applying fifteen cryopreservation or lyophilization conditions. After three, six and twelve months of preservation, the viability (via live-dead flow cytometry) and culturability (via most-probable number analysis and plating) of the cells were assessed. All strains could be cryopreserved without a significant loss in culturability using 1% trehalose in 10-fold diluted TSB (TT) as preservation medium and 5% DMSO as cryoprotectant. Several other cryopreservation and lyophilization conditions, all of which involved the use of TT medium, also allowed successful preservation but showed a considerable loss in culturability. We demonstrate here that most of these non-culturables survived preservation according to viability assessment indicating that preservation induces a viable but non-culturable (VBNC) state in a significant fraction of cells. Since this state is reversible, these findings have major implications shifting the emphasis from survival to revival of cells in a preservation protocol. We showed that MOB cells could be significantly resuscitated from the VBNC state using the TT preservation medium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.