ArticleGenesis (New York, N.Y. : 2000)2012
Generation of Nkx2.2:lacZ mice using recombination-mediated cassette exchange technology.
Article in Genesis (New York, N.Y. : 2000), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Human NKX2.2 influences islet endocrine cell fate choices through regulation of WNT pathway genes.bioRxiv : the preprint server for biology · 2025Article
- NKX2.2 and KLF4 cooperate to regulate α-cell identity.Genes & development · 2025Article
- NKX2-2 based nuclei sorting on frozen human archival pancreas enables the enrichment of islet endocrine populations for single-nucleus RNA sequencing.BMC genomics · 2024Article
- Major β cell-specific functions of NKX2.2 are mediated via the NK2-specific domain.Genes & development · 2023Article
- Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs.Scientific reports · 2018Article
- Article
- Pancreatic β cell identity requires continual repression of non-β cell programs.The Journal of clinical investigation · 2017Article
- The novel enterochromaffin marker Lmx1a regulates serotonin biosynthesis in enteroendocrine cell lineages downstream of Nkx2.2.Development (Cambridge, England) · 2016Article
- Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Pancreatic islet cell development and regeneration.Current opinion in endocrinology, diabetes, and obesity · 2015Review
- Nkx2.2:Cre knock-in mouse line: a novel tool for pancreas- and CNS-specific gene deletion.Genesis (New York, N.Y. : 2000) · 2013Article
- Generation of mice encoding a conditional allele of Nkx2.2.Transgenic research · 2013Article
- Regulation of Neurod1 contributes to the lineage potential of Neurogenin3+ endocrine precursor cells in the pancreas.PLoS genetics · 2013Article
- Ghrelin expression in the mouse pancreas defines a unique multipotent progenitor population.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Nkx2.2 encodes a homeodomain transcription factor required for the correct specification and/or differentiation of cells in the pancreas, intestine, and central nervous system (CNS). To follow the fate of cells deleted for Nkx2.2 within these tissues, we generated Nkx2.2:lacZ knockin mice using a recombination-mediated cassette exchange (RMCE) approach. Expression analysis of lacZ and/or β-galactosidase in Nkx2.2(lacZ/+) heterozygote embryos and adults demonstrates that lacZ faithfully recapitulates endogenous Nkx2.2 expression. Furthermore, the Nkx2.2(lacZ/lacZ) homozygous embryos display phenotypes indistinguishable from the previously characterized Nkx2.2(-/-) strain. LacZ expression analyses in the Nkx2.2(lacZ/lacZ) homozygous embryos indicate that Nkx2.2-expressing progenitor cells within the pancreas are generated in their normal numbers and are not mislocalized within the pancreatic ductal epithelium or developing islets. In the CNS of Nkx2.2(lacZ/lacZ) embryos, LacZ-expressing cells within the ventral P3 progenitor domain display different migration properties depending on the developmental stage and their respective differentiation potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.