ArticleThe Journal of biological chemistry2012
Characterization of gut-associated cathepsin D hemoglobinase from tick Ixodes ricinus (IrCD1).
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- RNAi-mediated knockdown of the poultry red mite cathepsin D-1 impacts haemoglobin digestion.Parasites & vectors · 2026Article
- An Unusual Two-Domain Thyropin from Tick Saliva: NMR Solution Structure and Highly Selective Inhibition of Cysteine Cathepsins Modulated by Glycosaminoglycans.International journal of molecular sciences · 2024Article
- Insight Into the Dynamics of the Ixodes ricinus Nymphal Midgut Proteome.Molecular & cellular proteomics : MCP · 2023Article
- The biotechnological potential of proteases from hematophagous arthropod vectors.Frontiers in cellular and infection microbiology · 2023Article
- Multiplex substrate profiling by mass spectrometry for proteases.Methods in enzymology · 2023Article
- Prediction and validation of cross-protective candidate antigen of Hyalomma asiaticum cathepsin L between H. asiaticum and H. anatolicum.Experimental & applied acarology · 2022Article
- Mialostatin, a Novel Midgut Cystatin fromInternational journal of molecular sciences · 2021Article
- Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling.BMC genomics · 2021Article
- Catalogue of stage-specific transcripts in Ixodes ricinus and their potential functions during the tick life-cycle.Parasites & vectors · 2020Article
- Blood Digestion by Trypsin-Like Serine Proteases in the Replete Lyme Disease Vector Tick,Insects · 2020Article
- Cathepsin L-a novel cysteine protease from Haemaphysalis flava Neumann, 1897.Parasitology research · 2019Article
- Novel pseudo-aspartic peptidase from the midgut of the tick Rhipicephalus microplus.Scientific reports · 2019Article
- SmSP2: A serine protease secreted by the blood fluke pathogen Schistosoma mansoni with anti-hemostatic properties.PLoS neglected tropical diseases · 2018Article
- Proteases of haematophagous arthropod vectors are involved in blood-feeding, yolk formation and immunity - a review.Parasites & vectors · 2017Review
- RNA-seq analyses of the midgut from blood- and serum-fed Ixodes ricinus ticks.Scientific reports · 2016Article
- Anaplasma marginale Actively Modulates Vacuolar Maturation during Intracellular Infection of Its Tick Vector, Dermacentor andersoni.Applied and environmental microbiology · 2016Article
- Complementary Proteomic and Biochemical Analysis of Peptidases in Lobster Gastric Juice Uncovers the Functional Role of Individual Enzymes in Food Digestion.Marine biotechnology (New York, N.Y.) · 2016Article
- Integration of Ixodes ricinus genome sequencing with transcriptome and proteome annotation of the naïve midgut.BMC genomics · 2015Article
- An aspartic protease of the scabies mite Sarcoptes scabiei is involved in the digestion of host skin and blood macromolecules.PLoS neglected tropical diseases · 2013Article
- Substrate specificity of MarP, a periplasmic protease required for resistance to acid and oxidative stress in Mycobacterium tuberculosis.The Journal of biological chemistry · 2013Article
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
Abstract
To identify the gut-associated tick aspartic hemoglobinase, this work focuses on the functional diversity of multiple Ixodes ricinus cathepsin D forms (IrCDs). Out of three encoding genes representing Ixodes scapularis genome paralogs, IrCD1 is the most distinct enzyme with a shortened propeptide region and a unique pattern of predicted post-translational modifications. IrCD1 gene transcription is induced by tick feeding and is restricted to the gut tissue. The hemoglobinolytic role of IrCD1 was further supported by immunolocalization of IrCD1 in the vesicles of tick gut cells. Properties of recombinantly expressed rIrCD1 are consistent with the endo-lysosomal environment because the zymogen is autoactivated and remains optimally active in acidic conditions. Hemoglobin cleavage pattern of rIrCD1 is identical to that produced by the native enzyme. The preference for hydrophobic residues at the P1 and P1' position was confirmed by screening a novel synthetic tetradecapeptidyl substrate library. Outside the S1-S1' regions, rIrCD1 tolerates most amino acids but displays a preference for tyrosine at P3 and alanine at P2'. Further analysis of the cleavage site location within the peptide substrate indicated that IrCD1 is a true endopeptidase. The role in hemoglobinolysis was verified with RNAi knockdown of IrCD1 that decreased gut extract cathepsin D activity by >90%. IrCD1 was newly characterized as a unique hemoglobinolytic cathepsin D contributing to the complex intestinal proteolytic network of mainly cysteine peptidases in ticks.
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