ArticleJournal of microbiology (Seoul, Korea)2012
Remodeling of the glycosylation pathway in the methylotrophic yeast Hansenula polymorpha to produce human hybrid-type N-glycans.
Article in Journal of microbiology (Seoul, Korea), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- Recent progress on heterologous protein production in methylotrophic yeast systems.World journal of microbiology & biotechnology · 2024Review
- Humanization of Yeasts for Glycan-Type End-Products.Frontiers in microbiology · 2022Review
- Production of galactosylated complex-type N-glycans in glycoengineered Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2022Article
- Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up.Microbial cell factories · 2021Review
- Constructing a human complex type N-linked glycosylation pathway in Kluyveromyces marxianus.PloS one · 2020Article
- Advances in UsingFrontiers in bioengineering and biotechnology · 2019Review
- Structural analysis of N-/O-glycans assembled on proteins in yeasts.Journal of microbiology (Seoul, Korea) · 2018Article
- Genome and metabolic engineering in non-conventional yeasts: Current advances and applications.Synthetic and systems biotechnology · 2017Review
- Humanizing glycosylation pathways in eukaryotic expression systems.World journal of microbiology & biotechnology · 2017Review
- Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae.Microbial cell factories · 2016Article
- Expression of granulocyte colony stimulating factor (GCSF) in Hansenula polymorpha.Iranian journal of microbiology · 2016Article
- Hansenula polymorpha Hac1p Is Critical to Protein N-Glycosylation Activity Modulation, as Revealed by Functional and Transcriptomic Analyses.Applied and environmental microbiology · 2015Article
- Molecular characterization of hap complex components responsible for methanol-inducible gene expression in the methylotrophic yeast Candida boidinii.Eukaryotic cell · 2015Article
- Article
- Engineering yeast for producing human glycoproteins: where are we now?Future microbiology · 2015Article
- Novel cysteine-centered sulfur metabolic pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha.PloS one · 2014Article
- Expression of glycoproteins bearing complex human-like glycans with galactose terminal in Hansenula polymorpha.World journal of microbiology & biotechnology · 2013Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a step forward to achieve the generation of human complex-type N-glycans in the methylotrophic yeast Hansenula polymorpha, we here report the modification of the yeast glycosylation pathway by heterologous expression of the human gene encoding β-1,2-N-acetylglucosaminyltransferase I (GnTI). For the optimal expression of human GnTI in the yeast Golgi compartment, the catalytic domain of the GnTI was fused to various N-terminal leader sequences derived from the yeast type II membrane proteins. The vectors containing GnTI fusion constructs were introduced into the H. polymorpha och1Δ single and och1Δalg3Δ double mutant strains expressing the ER-targeted Aspergillus saitoi α-1,2 mannosidase, respectively. Both of the glycoengineered Hpoch1Δ and Hpoch1ΔHpalg3Δ strains were shown to produce successfully the hybrid-type glycans with a monoantennary N-acetylglucosamine (GlcNAc(1)Man(5)GlcNAc(2) and GlcNAc(1)Man(3)GlcNAc(2), respectively) by N-glycan profile analysis of cell wall proteins. Furthermore, by comparative analysis of byproduct formation and the glycosylation site occupancy, we propose that the Hpoch1Δ strain would be more suitable than the Hpoch1ΔHpalg3Δ strain as a host for the production of recombinant proteins with humanized glycans.
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