ArticleEuropean radiology2012
In vivo MRI discrimination between live and lysed iron-labelled cells using balanced steady state free precession.
Article in European radiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Novel non‑metal‑based contrast agents for MR imaging: Emerging approaches and clinical perspectives (Review).International journal of oncology · 2025Review
- Utilization of Computed Tomography Colonography (CTC) at a District General Hospital: A Retrospective Analysis.Cureus · 2025Article
- Micron-sized iron oxide particles for both MRI cell tracking and magnetic fluid hyperthermia treatment.Scientific reports · 2021Article
- Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting.Scientific reports · 2018Article
- Advances in Monitoring Cell-Based Therapies with Magnetic Resonance Imaging: Future Perspectives.International journal of molecular sciences · 2017Review
- Imaging transplanted stem cells in real time using an MRI dual-contrast method.Scientific reports · 2015Article
- Fluorine-19 MRI Contrast Agents for Cell Tracking and Lung Imaging.Magnetic resonance insights · 2015Review
- Extensive expansion of primary human gamma delta T cells generates cytotoxic effector memory cells that can be labeled with Feraheme for cellular MRI.Cancer immunology, immunotherapy : CII · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe goal of this study was to evaluate the ability of balanced steady state free precession (b-SSFP) magnetic resonance imaging sequence to distinguish between live and lysed iron-labelled cells.
methodsHuman breast cancer cells were labelled with iron oxide nanoparticles. Cells were lysed using sonication. Imaging was performed at 3 T. The timing parameters for b-SSFP and the number of iron-labelled cells in samples were varied to optimise the b-SSFP signal difference between live and lysed iron-labelled cell samples. For in vivo experiments, cells were mixed with Matrigel and implanted into nude mice. Three mice implanted with live labelled cancer cells were irradiated to validate this method.
resultsLysed iron-labelled cells have a significantly higher signal compared with live, intact iron-labelled cells in bSSFP images. The contrast between live and dead cells can be maximised by careful optimisation of timing parameters. A change in the b-SSFP signal was measured 6 days after irradiation, reflecting cell death in vivo. Histology confirmed the presence of dead cells in the implant.
conclusionsOur results show that the b-SSFP sequence can be optimised to allow for the discrimination of live iron-labelled cells and lysed iron-labelled cells in vitro and in vivo.
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