ArticleHeart rhythm2012
The voltage-sensitive dye di-4-ANEPPS slows conduction velocity in isolated guinea pig hearts.
Article in Heart rhythm, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Genetic background determines the severity of age-dependent cardiac structural abnormalities and arrhythmia susceptibility in Scn5a-1798insD mice.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2024Article
- Optical mapping of contracting hearts.The Journal of physiology · 2023Review
- High resolution optical mapping of cardiac electrophysiology in pre-clinical models.Scientific data · 2022Article
- Ultrafast four-dimensional imaging of cardiac mechanical wave propagation with sparse optoacoustic sensing.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- High heart rate associated early repolarization causes J-waves in both zebra finch and mouse.Physiological reports · 2021Article
- Di-4-ANEPPS Modulates Electrical Activity and Progress of Myocardial Ischemia in Rabbit Isolated Heart.Frontiers in physiology · 2021Article
- Cardiac optical mapping - State-of-the-art and future challenges.The international journal of biochemistry & cell biology · 2020Review
- Attenuating loss of cardiac conduction during no-flow ischemia through changes in perfusate sodium and calcium.American journal of physiology. Heart and circulatory physiology · 2020Article
- Optical Electrophysiology in the Developing Heart.Journal of cardiovascular development and disease · 2018Review
- Optimization of Fluorescent Labeling forBioMed research international · 2018Article
- TNFα Modulates Cardiac Conduction by Altering Electrical Coupling between Myocytes.Frontiers in physiology · 2017Article
- Diverse Fibrosis Architecture and Premature Stimulation Facilitate Initiation of Reentrant Activity Following Chronic Atrial Fibrillation.Journal of cardiovascular electrophysiology · 2015Article
- Role of Calcium-activated Potassium Channels in Atrial Fibrillation Pathophysiology and Therapy.Journal of cardiovascular pharmacology · 2015Review
- A century of optocardiography.IEEE reviews in biomedical engineering · 2014Review
- Absence of glucose transporter 4 diminishes electrical activity of mouse hearts during hypoxia.Experimental physiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundVoltage-sensitive dyes are important tools for mapping electrical activity in the heart. However, little is known about the effects of voltage-sensitive dyes on cardiac electrophysiology.
objectiveTo test the hypothesis that the voltage-sensitive dye di-4-ANEPPS modulates cardiac impulse propagation.
methodsElectrical and optical mapping experiments were performed in isolated Langendorff perfused guinea pig hearts. The effect of di-4-ANEPPS on conduction velocity and anisotropy of propagation was quantified. HeLa cells expressing connexin 43 were used to evaluate the effect of di-4-ANEPPS on gap junctional conductance.
resultsIn electrical mapping experiments, di-4-ANEPPS (7.5 μM) was found to decrease both longitudinal and transverse conduction velocities significantly compared with control. No change in the anisotropy of propagation was observed. Similar results were obtained in optical mapping experiments. In these experiments, the effect of di-4-ANEPPS was dose dependent. di-4-ANEPPS had no detectable effect on connexin 43-mediated gap junctional conductance in transfected HeLa cells.
conclusionOur results demonstrate that the voltage-sensitive dye di-4-ANEPPS directly and dose-dependently modulates cardiac impulse propagation. The effect is not likely mediated by connexin 43 inhibition. Our results highlight an important caveat that should be taken into account when interpreting data obtained using di-4-ANEPPS in cardiac preparations.
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