ArticleThe journal of physical chemistry. B2012
Multiphasic DNA adsorption to silica surfaces under varying buffer, pH, and ionic strength conditions.
Article in The journal of physical chemistry. B, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed, 124 citations in OpenAlex.
- Detrimental effect of metal contaminants on forensic DNA analysis and strategies for mitigation.International journal of legal medicine · 2026Review
- Quantitative analysis of sequential nucleic acid elution from silica paramagnetic beads.The Analyst · 2026Article
- QuickConc: A Rapid, Efficient, and Power-Free eDNA Concentration Method With Cationic-Assisted Capture.Ecology and evolution · 2025Article
- A rapid and high-yield method for nucleic acid extraction.Scientific reports · 2025Article
- A primordial DNA store and compute engine.Nature nanotechnology · 2024Article
- Extracting regulatory active chromatin footprint from cell-free DNA.Communications biology · 2024Article
- Optimizing Messenger RNA Analysis Using Ultra-Wide Pore Size Exclusion Chromatography Columns.International journal of molecular sciences · 2024Article
- A high-performance polymer composite column for coronavirus nucleic acid purification.Scientific reports · 2024Article
- UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood.ACS applied materials & interfaces · 2023Article
- Selective isolation of hyaluronan by solid phase adsorption to silica.Analytical biochemistry · 2022Article
- Comparative Evaluation of Different Surface Coatings of FeInternational journal of molecular sciences · 2022Article
- The Multiple States of Environmental DNA and What Is Known about Their Persistence in Aquatic Environments.Environmental science & technology · 2022Review
- Long-term stabilization of DNA at room temperature using a one-step microwave assisted process.Emergent materials · 2022Article
- The Role of Extracellular DNA in Microbial Attachment to Oxidized Silicon Surfaces in the Presence of CaLangmuir : the ACS journal of surfaces and colloids · 2021Article
- Rapid and simple single-chamber nucleic acid detection system prepared through nature-inspired surface engineering.Theranostics · 2021Article
- Ultrasensitive hybridization capture: Reliable detection of <1 copy/mL short cell-free DNA from large-volume urine samples.PloS one · 2021Article
- Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane.Scientific reports · 2020Article
- SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP.Viruses · 2020Article
- Enhanced detection of cell-free DNA (cfDNA) enables its use as a reliable biomarker for diagnosis and prognosis of gastric cancer.PloS one · 2020Article
- UltraPrep is a scalable, cost-effective, bead-based method for purifying cell-free DNA.PloS one · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Reversible interactions between DNA and silica are utilized in the solid phase extraction and purification of DNA from complex samples. Chaotropic salts commonly drive DNA binding to silica but inhibit DNA polymerase amplification. We studied DNA adsorption to silica using conditions with or without chaotropic salts through bulk depletion and quartz crystal microbalance (QCM) experiments. While more DNA adsorbed to silica using chaotropic salts, certain buffer conditions without chaotropic salts yielded a similar amount of eluted DNA. QCM results indicate that under stronger adsorbing conditions the adsorbed DNA layer is initially rigid but becomes viscoelastic within minutes. These results qualitatively agreed with a mathematical model for a multiphasic adsorption process. Buffer conditions that do not require chaotropic salts can simplify protocols for nucleic acid sample preparation. Understanding how DNA adsorbs to silica can help optimize nucleic acid sample preparation for clinical diagnostic and research applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.