ArticlePloS one2012
IL-6 and mouse oocyte spindle.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
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- Single-cell analysis of ovarian myeloid cells identifies age-associated changes in macrophages and signaling dynamics†.Biology of reproduction · 2025Article
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- Unravelling the Signature Follicular Fluid Metabolites in Dairy Cattle Follicles Growing Under Negative Energy Balance: An In Vitro Approach.International journal of molecular sciences · 2024Article
- Association between dietary inflammation index and female infertility from National Health and Nutrition Examination Survey: 2013-2018.Frontiers in endocrinology · 2024Article
- Chito-oligosaccharides and macrophages have synergistic effects on improving ovarian stem cells function by regulating inflammatory factors.Journal of ovarian research · 2023Article
- Serum C-reactive protein levels are associated with clinical pregnancy rate afterFrontiers in endocrinology · 2023Article
- Potential Role of Zinc in the COVID-19 Disease Process and its Probable Impact on Reproduction.Reproductive sciences (Thousand Oaks, Calif.) · 2022Review
- Transcriptome Analysis Reveals the Mechanism of Natural Ovarian Ageing.Frontiers in endocrinology · 2022Article
- Getting to Know Endometriosis-Related Infertility Better: A Review on How Endometriosis Affects Oocyte Quality and Embryo Development.Biomedicines · 2021Review
- Osteoarthritis Affects Mammalian Oogenesis: Effects of Collagenase-Induced Osteoarthritis on Oocyte Cytoskeleton in a Mouse Model.International journal of inflammation · 2021Article
- Interleukin 6 in follicular fluid reduces embryo fragmentation and improves the clinical pregnancy rate.Journal of assisted reproduction and genetics · 2020Article
- Inflammaging is associated with shifted macrophage ontogeny and polarization in the aging mouse ovary.Reproduction (Cambridge, England) · 2020Article
- Inflammation: friend or foe of bovine reproduction?Animal reproduction · 2019Article
- Cyclophosphamide and acrolein induced oxidative stress leading to deterioration of metaphase II mouse oocyte quality.Free radical biology & medicine · 2017Article
- Galactose and its Metabolites Deteriorate Metaphase II Mouse Oocyte Quality and Subsequent Embryo Development by Disrupting the Spindle Structure.Scientific reports · 2017Article
- Clinical management of endometriosis-associated infertility.Reproductive medicine and biology · 2016Review
- The Impact of Myeloperoxidase and Activated Macrophages on Metaphase II Mouse Oocyte Quality.PloS one · 2016Article
- Effect of induced peritoneal endometriosis on oocyte and embryo quality in a mouse model.Journal of assisted reproduction and genetics · 2015Article
- Impact of hydrogen peroxide-driven Fenton reaction on mouse oocyte quality.Free radical biology & medicine · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interleukin 6 (IL-6) is considered a major indicator of the acute-phase inflammatory response. Endometriosis and pelvic inflammation, diseases that manifest elevated levels of IL-6, are commonly associated with higher infertility. However, the mechanistic link between elevated levels of IL-6 and poor oocyte quality is still unclear. In this work, we explored the direct role of this cytokine as a possible mediator for impaired oocyte spindle and chromosomal structure, which is a critical hurdle in the management of infertility. Metaphase-II mouse oocytes were exposed to recombinant mouse IL-6 (50, 100 and 200 ng/mL) for 30 minutes and subjected to indirect immunofluorescent staining to identify alterations in the microtubule and chromosomal alignment compared to untreated controls. The deterioration in microtubule and chromosomal alignment were evaluated utilizing both fluorescence and confocal microscopy, and were quantitated with a previously reported scoring system. Our results showed that IL-6 caused a dose-dependent deterioration in microtubule and chromosomal alignment in the treated oocytes as compared to the untreated group. Indeed, IL-6 at a concentration as low as 50 ng/mL caused deterioration in the spindle structure in 60% of the oocytes, which increased significantly (P<0.0001) as IL-6 concentration was increased. In conclusion, elevated levels of IL-6 associated with endometriosis and pelvic inflammation may reduce the fertilizing capacity of human oocyte through a mechanism that involves impairment of the microtubule and chromosomal structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.