ArticlePloS one2012
TRF2 controls telomeric nucleosome organization in a cell cycle phase-dependent manner.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Structural organization and function of telomeric chromatin.Nature cell biology · 2026Review
- Structural biology of shelterin and telomeric chromatin: the pieces and an unfinished puzzle.Biochemical Society transactions · 2024Review
- The shelterin component TRF2 mediates columnar stacking of human telomeric chromatin.The EMBO journal · 2024Article
- The human telomeric proteome during telomere replication.Nucleic acids research · 2021Article
- Shaping human telomeres: from shelterin and CST complexes to telomeric chromatin organization.Nature reviews. Molecular cell biology · 2021Review
- Epigenetic aspects of telomeric chromatin in Arabidopsis thaliana.BMB reports · 2019Review
- Emerging roles of telomeric chromatin alterations in cancer.Journal of experimental & clinical cancer research : CR · 2019Review
- Telomere length-dependent transcription and epigenetic modifications in promoters remote from telomere ends.PLoS genetics · 2018Article
- Local enrichment of HP1alpha at telomeres alters their structure and regulation of telomere protection.Nature communications · 2018Article
- Life and cancer without telomerase: ALT and other strategies for making sure ends (don't) meet.Critical reviews in biochemistry and molecular biology · 2017Review
- TRF2 Protein Interacts with Core Histones to Stabilize Chromosome Ends.The Journal of biological chemistry · 2016Article
- Guarding chromosomes from oxidative DNA damage to the very end.Acta biochimica et biophysica Sinica · 2016Review
- Telomere-binding protein regulates the chromosome ends through the interaction with histone deacetylases in Arabidopsis thaliana.Nucleic acids research · 2016Article
- Article
- TRF2-Mediated Control of Telomere DNA Topology as a Mechanism for Chromosome-End Protection.Molecular cell · 2016Article
- Hypothesis: Paralog Formation from Progenitor Proteins and Paralog Mutagenesis Spur the Rapid Evolution of Telomere Binding Proteins.Frontiers in genetics · 2016Article
- DNA excision repair at telomeres.DNA repair · 2015Review
- SLX4 contributes to telomere preservation and regulated processing of telomeric joint molecule intermediates.Nucleic acids research · 2015Article
- TRF1 and TRF2 binding to telomeres is modulated by nucleosomal organization.Nucleic acids research · 2015Article
- AKTIP/Ft1, a New Shelterin-Interacting Factor Required for Telomere Maintenance.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian telomeres stabilize chromosome ends as a result of their assembly into a peculiar form of chromatin comprising a complex of non-histone proteins named shelterin. TRF2, one of the shelterin components, binds to the duplex part of telomeric DNA and is essential to fold the telomeric chromatin into a protective cap. Although most of the human telomeric DNA is organized into tightly spaced nucleosomes, their role in telomere protection and how they interplay with telomere-specific factors in telomere organization is still unclear. In this study we investigated whether TRF2 can regulate nucleosome assembly at telomeres.By means of chromatin immunoprecipitation (ChIP) and Micrococcal Nuclease (MNase) mapping assay, we found that the density of telomeric nucleosomes in human cells was inversely proportional to the dosage of TRF2 at telomeres. This effect was not observed in the G1 phase of the cell cycle but appeared coincident of late or post-replicative events. Moreover, we showed that TRF2 overexpression altered nucleosome spacing at telomeres increasing internucleosomal distance. By means of an in vitro nucleosome assembly system containing purified histones and remodeling factors, we reproduced the short nucleosome spacing found in telomeric chromatin. Importantly, when in vitro assembly was performed in the presence of purified TRF2, nucleosome spacing on a telomeric DNA template increased, in agreement with in vivo MNase mapping.Our results demonstrate that TRF2 negatively regulates the number of nucleosomes at human telomeres by a cell cycle-dependent mechanism that alters internucleosomal distance. These findings raise the intriguing possibility that telomere protection is mediated, at least in part, by the TRF2-dependent regulation of nucleosome organization.
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