ArticleJournal of biomedicine & biotechnology2012
A laccase with HIV-1 reverse transcriptase inhibitory activity from the broth of mycelial culture of the mushroom Lentinus tigrinus.
Article in Journal of biomedicine & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 25 citations in OpenAlex.
- Modern Pro-Health Applications of Medicinal Mushrooms: Insights into theMolecules (Basel, Switzerland) · 2025Review
- Mushrooms as future generation healthy foods.Frontiers in nutrition · 2022Review
- Natural Products with Inhibitory Activity against Human Immunodeficiency Virus Type 1.Advances in virology · 2021Review
- Anti-viral activity of culinary and medicinal mushroom extracts against dengue virus serotype 2: an in-vitro study.BMC complementary and alternative medicine · 2019Article
- Molecular cloning, expression and characterization of poxa1b gene from Pleurotus ostreatus.Molecular biology reports · 2019Article
- Purification and characterization of laccase from Marasmius species BBKAV79 and effective decolorization of selected textile dyes.3 Biotech · 2016Article
- How to enjoy laccases.Cellular and molecular life sciences : CMLS · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 59 kDa laccase with inhibitory activity against HIV-1 reverse transcriptase (IC(50) = 2.4 μM) was isolated from the broth of mycelial culture of the mushroom Lentinus tigrinus. The isolation procedure involved ion exchange chromatography on DEAE-cellulose and CM-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The laccase was adsorbed on both types of ion exchangers. About 95-fold purification was achieved with a 25.9% yield of the enzyme. The procedure resulted in a specific enzyme activity of 76.6 U/mg. Its N-terminal amino acid sequence was GIPDLHDLTV, which showed little similarity to other mushroom laccase and other Lentinus tigrinus strain laccase. Its characteristics were different from previously reported laccase of other Lentinus tigrinus strain. Maximal laccase activity was observed at a pH of 4 and at a temperature of 60°C, respectively. This study yielded the information about the potentially exploitable activities of Lentinus tigrinus laccase.
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