ArticleJournal of clinical microbiology2012
Accurate identification of Candida parapsilosis (sensu lato) by use of mitochondrial DNA and real-time PCR.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Catechin Augments the Antifungal Efficacy of Fluconazole AgainstInternational journal of molecular sciences · 2026Article
- Article
- The Emerging PathogenInfection and drug resistance · 2024Review
- Recombinase Polymerase Amplification Assay with Lateral Flow Strips for Rapid Detection of Candidiasis Due to Candida parapsilosis.Current microbiology · 2023Article
- Establishment of a rapid diagnosis method forFrontiers in cellular and infection microbiology · 2022Article
- Clonal Candidemia Outbreak byFrontiers in cellular and infection microbiology · 2021Article
- Microsatellite genotyping of clinical Candida parapsilosis isolates.Current medical mycology · 2017Article
- Comparison of four molecular approaches to identify Candida parapsilosis complex species.Memorias do Instituto Oswaldo Cruz · 2017Article
- Outbreak of candidemia caused by fluconazole resistant Candida parapsilosis strains in an intensive care unit.BMC infectious diseases · 2016Article
- Evolution and Application of Inteins inFrontiers in microbiology · 2016Review
- Candida parapsilosis Resistance to Fluconazole: Molecular Mechanisms and In Vivo Impact in Infected Galleria mellonella Larvae.Antimicrobial agents and chemotherapy · 2015Article
- Genetic diversity of medically important and emerging Candida species causing invasive infection.BMC infectious diseases · 2015Article
- Article
- Article
- Development of two molecular approaches for differentiation of clinically relevant yeast species closely related to Candida guilliermondii and Candida famata.Journal of clinical microbiology · 2014Article
- Identification and differentiation of Candida parapsilosis complex species by use of exon-primed intron-crossing PCR.Journal of clinical microbiology · 2014Article
- Breakthrough candidemia due to multidrug-resistant Candida glabrata during prophylaxis with a low dose of micafungin.Antimicrobial agents and chemotherapy · 2014Article
- Analysis of inteins in the Candida parapsilosis complex for simple and accurate species identification.Journal of clinical microbiology · 2013Article
- Molecular identification, antifungal susceptibility profile, and biofilm formation of clinical and environmental Rhodotorula species isolates.Antimicrobial agents and chemotherapy · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Candida parapsilosis is the Candida species isolated the second most frequently from blood cultures in South America and some European countries, such as Spain. Since 2005, this species has been considered a complex of 3 closely related species: C. parapsilosis, Candida metapsilosis, and Candida orthopsilosis. Here, we describe a real-time TaqMan-MGB PCR assay, using mitochondrial DNA (mtDNA) as the target, which readily distinguishes these 3 species. We first used comparative genomics to locate syntenic regions between these 3 mitochondrial genomes and then selected NADH5 as the target for the real-time PCR assay. Probes were designed to include a combination of different single-nucleotide polymorphisms that are able to differentiate each species within the C. parapsilosis complex. This new methodology was first tested using mtDNA and then genomic DNA from 4 reference and 5 clinical strains. For assay validation, a total of 96 clinical isolates and 4 American Type Culture Collection (ATCC) isolates previously identified by internal transcribed spacer (ITS) ribosomal DNA (rDNA) sequencing were tested. Real-time PCR using genomic DNA was able to differentiate the 3 species with 100% accuracy. No amplification was observed when DNA from other species was used as the template. We observed 100% congruence with ITS rDNA sequencing identification, including for 30 strains used in blind testing. This novel method allows a quick and accurate intracomplex identification of C. parapsilosis and saves time compared with sequencing, which so far has been considered the "gold standard" for Candida yeast identification. In addition, this assay provides a useful tool for epidemiological and clinical studies of these emergent species.
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