ArticleThe Journal of biological chemistry2012
Redox regulation of carbonic anhydrases via thioredoxin in chloroplast of the marine diatom Phaeodactylum tricornutum.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- Enhanced sequestration of carbon dioxide into calcium carbonate using pressure and a carbonic anhydrase from alkaliphilicEngineering in life sciences · 2022Article
- Review
- Thiol Redox Regulation of Plant β-Carbonic Anhydrase.Biomolecules · 2020Article
- Redox Conformation-Specific Protein-Protein Interactions of the 2-Cysteine Peroxiredoxin in Arabidopsis.Antioxidants (Basel, Switzerland) · 2020Article
- Insights on the Functions and Ecophysiological Relevance of the Diverse Carbonic Anhydrases in Microalgae.International journal of molecular sciences · 2020Review
- Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity.The Plant cell · 2020Review
- Regulation of Carbon Metabolism by Environmental Conditions: A Perspective From Diatoms and Other Chromalveolates.Frontiers in plant science · 2020Review
- Mechanisms of carbon dioxide acquisition and COPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Evolutionarily distinct strategies for the acquisition of inorganic carbon from seawater in marine diatoms.Journal of experimental botany · 2017Article
- Structural insights into the LCIB protein family reveals a new group of β-carbonic anhydrases.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Light and CO2/cAMP Signal Cross Talk on the Promoter Elements of Chloroplastic β-Carbonic Anhydrase Genes in the Marine Diatom Phaeodactylum tricornutum.Plant physiology · 2016Article
- Localization of putative carbonic anhydrases in the marine diatom, Thalassiosira pseudonana.Photosynthesis research · 2014Article
- A chloroplast pump model for the CO2 concentrating mechanism in the diatom Phaeodactylum tricornutum.Photosynthesis research · 2014Article
- High light acclimation in the secondary plastids containing diatom Phaeodactylum tricornutum is triggered by the redox state of the plastoquinone pool.Plant physiology · 2013Article
- Blue light is essential for high light acclimation and photoprotection in the diatom Phaeodactylum tricornutum.Journal of experimental botany · 2013Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thioredoxins (Trxs) are important regulators of photosynthetic fixation of CO(2) and nitrogen in plant chloroplasts. To date, they have been considered to play a minor role in controlling the Calvin cycle in marine diatoms, aquatic primary producers, although diatoms possess a set of plastidic Trxs. In this study we examined the influences of the redox state and the involvement of Trxs in the enzymatic activities of pyrenoidal carbonic anhydrases, PtCA1 and PtCA2, in the marine diatom Phaeodactylum tricornutum. The recombinant mature PtCA1 and -2 (mPtCA1 and -2) were completely inactivated following oxidation by 50 μm CuCl(2), whereas DTT activated CAs in a concentration-dependent manner. The maximum activity of mPtCAs in the presence of 6 mm reduced DTT increased significantly by addition of 10 μm Trxs from Arabidopsis thaliana (AtTrx-f2 and -m2) and 5 μm Trxs from P. tricornutum (PtTrxF and -M). Analyses of mPtCA activation by Trxs in the presence of DTT revealed that the maximum mPtCA1 activity was enhanced ∼3-fold in the presence of Trx, whereas mPtCA2 was only weakly activated by Trxs, and that PtTrxs activate PtCAs more efficiently compared with AtTrxs. Site-directed mutagenesis of potential disulfide-forming cysteines in mPtCA1 and mPtCA2 resulted in a lack of oxidative inactivation of both mPtCAs. These results reveal the first direct evidence of a target of plastidic Trxs in diatoms, indicating that Trxs may participate in the redox control of inorganic carbon flow in the pyrenoid, a focal point of the CO(2)-concentrating mechanism.
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