ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2012
Identification of FAD2 and FAD3 genes in Brassica napus genome and development of allele-specific markers for high oleic and low linolenic acid contents.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 147 citations in OpenAlex.
- Towards premium rapeseed oil: leveraging allelic variation in BnaFAD2.A5, BnaFAD3.A4 and BnaFAD3.C4 for molecular design breeding.BMC plant biology · 2026Article
- Molecular breeding approaches for the improvement of oil content and fatty acid composition in exotic-derived maize germplasm.The New phytologist · 2025Article
- Identification of transcription factor BnHDG4-A08 as a novel candidate associated with the accumulation of oleic, linoleic, linolenic, and erucic acid in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Comparative Transcriptomics Uncovers Upstream Factors RegulatingPlants (Basel, Switzerland) · 2024Article
- Improving linolenic acid content in rapeseed oil by overexpression ofMolecular breeding : new strategies in plant improvement · 2024Article
- Targeted mutagenesis of flavonoid biosynthesis pathway genes reveals functional divergence in seed coat colour, oil content and fatty acid composition in Brassica napus L.Plant biotechnology journal · 2024Article
- A chromosome-level genome assembly provides insights intoHorticulture research · 2023Article
- A novel type of Brassica napus with higher stearic acid in seeds developed through genome editing of BnaSAD2 family.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Characterization of novel loci controlling seed oil content in Brassica napus by marker metabolite-based multi-omics analysis.Genome biology · 2023Article
- Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance.BMC plant biology · 2023Article
- Sequencing of Camelina neglecta, a diploid progenitor of the hexaploid oilseed Camelina sativa.Plant biotechnology journal · 2023Article
- CRISPR/Cas9-Mediated Gene Editing ofGenes · 2022Article
- Clone and Function Verification of the OPR gene in Brassica napus Related to Linoleic Acid Synthesis.BMC plant biology · 2022Article
- Multi-omics analysis dissects the genetic architecture of seed coat content in Brassica napus.Genome biology · 2022Article
- Increase in alpha-linolenic acid content by simultaneous expression of fatty acid metabolism genes in Sesame (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Overexpression ofGenes · 2022Article
- Critical metabolic pathways and genes cooperate for epoxy fatty acid-enriched oil production in developing seeds of Vernonia galamensis, an industrial oleaginous plant.Biotechnology for biofuels and bioproducts · 2022Article
- Peach fruit PpNAC1 activatesHorticulture research · 2022Article
- CRISPR/Cas9-mediated editing of double loci ofFrontiers in plant science · 2022Article
- Advanced domestication: harnessing the precision of gene editing in crop breeding.Plant biotechnology journal · 2021Review
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8 authors at 1 institution in 1 country.
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Abstract
Modification of oleic acid (C18:1) and linolenic acid (C18:3) contents in seeds is one of the major goals for quality breeding after removal of erucic acid in oilseed rape (Brassica napus). The fatty acid desaturase genes FAD2 and FAD3 have been shown as the major genes for the control of C18:1 and C18:3 contents. However, the genome structure and locus distributions of the two gene families in amphidiploid B. napus are still not completely understood to date. In the present study, all copies of FAD2 and FAD3 genes in the A- and C-genome of B. napus and its two diploid progenitor species, Brassica rapa and Brassica oleracea, were identified through bioinformatic analysis and extensive molecular cloning. Two FAD2 genes exist in B. rapa and B. oleracea, and four copies of FAD2 genes exist in B. napus. Three and six copies of FAD3 genes were identified in diploid species and amphidiploid species, respectively. The genetic control of high C18:1 and low C18:3 contents in a double haploid population was investigated through mapping of the quantitative trait loci (QTL) for the traits and the molecular cloning of the underlying genes. One major QTL of BnaA.FAD2.a located on A5 chromosome was responsible for the high C18:1 content. A deleted mutation in the BnaA.FAD2.a locus was uncovered, which represented a previously unidentified allele for the high oleic variation in B. napus species. Two major QTLs on A4 and C4 chromosomes were found to be responsible for the low C18:3 content in the DH population as well as in SW Hickory. Furthermore, several single base pair changes in BnaA.FAD3.b and BnaC.FAD3.b were identified to cause the phenotype of low C18:3 content. Based on the results of genetic mapping and identified sequences, allele-specific markers were developed for FAD2 and FAD3 genes. Particularly, single-nucleotide amplified polymorphisms markers for FAD3 alleles were demonstrated to be a reliable type of SNP markers for unambiguous identification of genotypes with different content of C18:3 in amphidiploid B. napus.
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