ArticleThe ISME journal2012
Functional metagenomics reveals novel salt tolerance loci from the human gut microbiome.
Article in The ISME journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- Genomic and Phenotypic Bases of Salt Tolerance in Sinorhizobium meliloti: Candidate Traits for Bioinoculant Development Addressing Saline Soils.Microbial biotechnology · 2026Article
- ABC-type salt tolerance transporter genes are abundant and mutually shared among the microorganisms of the hypersaline Sambhar Lake.Extremophiles : life under extreme conditions · 2025Article
- An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food-Microbiota-Host Interactions in the Human Gut.International journal of molecular sciences · 2023Review
- Vitamins as regulators of calcium-containing kidney stones - new perspectives on the role of the gut microbiome.Nature reviews. Urology · 2023Review
- Effects of rice blast biocontrol strainFrontiers in microbiology · 2023Article
- Insights into vitamin A in bladder cancer, lack of attention to gut microbiota?Frontiers in immunology · 2023Review
- Functional metagenomic libraries generated from anthropogenically impacted environments reveal importance of metabolic genes in biocide and antibiotic resistance.Current research in microbial sciences · 2023Article
- A plasmid network from the gut microbiome of semi-isolated human groups reveals unique and shared metabolic and virulence traits.Scientific reports · 2022Article
- Isolation of novel cold-tolerance genes from rhizosphere microorganisms of Antarctic plants by functional metagenomics.Frontiers in microbiology · 2022Article
- Mining for Perchlorate Resistance Genes in Microorganisms From Sediments of a Hypersaline Pond in Atacama Desert, Chile.Frontiers in microbiology · 2021Article
- Isolation of gene conferring salt tolerance from halophilic bacteria of Lunsu, Himachal Pradesh, India.Journal, genetic engineering & biotechnology · 2020Article
- Metagenomics-Guided Discovery of Potential Bacterial Metallothionein Genes from the Soil Microbiome That Confer Cu and/or Cd Resistance.Applied and environmental microbiology · 2020Article
- Molecular and Biochemical Characterization of Salt-Tolerant Trehalose-6-Phosphate Hydrolases Identified by Screening and Sequencing Salt-Tolerant Clones From the Metagenomic Library of the Gastrointestinal Tract.Frontiers in microbiology · 2020Article
- Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments.Frontiers in microbiology · 2020Article
- Metagenomic Profiling of Soil Microbes to Mine Salt Stress Tolerance Genes.Frontiers in microbiology · 2018Article
- Carriage of λ Latent Virus Is Costly for Its Bacterial Host due to Frequent Reactivation in Monoxenic Mouse Intestine.PLoS genetics · 2016Article
- Functional metagenomic discovery of bacterial effectors in the human microbiome and isolation of commendamide, a GPCR G2A/132 agonist.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics.Molecular systems biology · 2015Article
- Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment.Frontiers in microbiology · 2015Article
- Effects of transportation stress and addition of salt to transport water on the skin mucosal homeostasis of rainbow trout (Aquaculture (Amsterdam, Netherlands) · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metagenomics is a powerful tool that allows for the culture-independent analysis of complex microbial communities. One of the most complex and dense microbial ecosystems known is that of the human distal colon, with cell densities reaching up to 10(12) per gram of faeces. With the majority of species as yet uncultured, there are an enormous number of novel genes awaiting discovery. In the current study, we conducted a functional screen of a metagenomic library of the human gut microbiota for potential salt-tolerant clones. Using transposon mutagenesis, three genes were identified from a single clone exhibiting high levels of identity to a species from the genus Collinsella (closest relative being Collinsella aerofaciens) (COLAER_01955, COLAER_01957 and COLAER_01981), a high G+C, Gram-positive member of the Actinobacteria commonly found in the human gut. The encoded proteins exhibit a strong similarity to GalE, MurB and MazG. Furthermore, pyrosequencing and bioinformatic analysis of two additional fosmid clones revealed the presence of an additional galE and mazG gene, with the highest level of genetic identity to Akkermansia muciniphila and Eggerthella sp. YY7918, respectively. Cloning and heterologous expression of the genes in the osmosensitive strain, Escherichia coli MKH13, resulted in increased salt tolerance of the transformed cells. It is hoped that the identification of atypical salt tolerance genes will help to further elucidate novel salt tolerance mechanisms, and will assist our increased understanding how resident bacteria cope with the osmolarity of the gastrointestinal tract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.