ArticleThe ISME journal2012
Leaf microbiota in an agroecosystem: spatiotemporal variation in bacterial community composition on field-grown lettuce.
Article in The ISME journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 185 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
185 citing papers in PubMed, 1 synthesis or guideline pooled it, 460 citations in OpenAlex.
- Pooled it
- Review
- Commercial washing and storage over shelf life impact bacterial communities more than the fungal communities on baby spinach.Microbiology spectrum · 2026Article
- Host genotype Impacts the Assembly and Functional Potential of Bacterial Communities in the Spinach Phyllosphere Within Commercial Organic Farming Systems.Microbial ecology · 2026Article
- Nutrient content of grass influences microbial communities in the phyllosphere more than climate change.Applied microbiology and biotechnology · 2026Article
- Plant phenotypic differentiation outweighs genetic variation in shaping the lettuce leaf microbiota.Environmental microbiome · 2026Article
- Shredder identity matters: taxon-specific gut microbiota in aquatic shredders drives bacterial diversity during leaf breakdown in streams.ISME communications · 2026Article
- Linking the edible plant microbiome and human gut microbiome.Gut microbes · 2025Review
- Evaluating the Capability ofPlants (Basel, Switzerland) · 2025Article
- Distinct bacterial communities affiliated with two types of shredder-produced particles in streams.FEMS microbiology ecology · 2025Article
- Long-term impacts of untreated dairy manure on the microbiome and Shiga toxin-producingApplied and environmental microbiology · 2025Article
- Differentiation and Interconnection of the Bacterial Community Associated withPlants (Basel, Switzerland) · 2025Article
- The Impact of Tetracycline on the Soil Microbiome and the Rhizosphere of Lettuce (International journal of molecular sciences · 2025Article
- Dynamic quinone repertoire accompanied the diversification of energy metabolism in Pseudomonadota.The ISME journal · 2025Article
- Niche-specific maize microbiomes enhance productivity and nitrogen uptake under intercropping.Frontiers in microbiology · 2025Article
- Insights into the association ofFrontiers in plant science · 2025Article
- Differential Responses of Methylobacterium and Sphingomonas Species to Multispecies Interactions in the Phyllosphere.Environmental microbiology · 2025Article
- Bacterial abundance and co-acclimation in mangrove rhizosphere and non-rhizosphere soils under pyrene stress.Frontiers in microbiology · 2025Article
- The phyllosphere of Nigerian medicinal plants, Euphorbia lateriflora and Ficus thonningii is inhabited by a specific microbiota.Scientific reports · 2024Article
- Aggregative Swab Sampling Method for Romaine Lettuce Show Similar Quality and Safety Indicators and Microbial Profiles Compared to Composite Produce Leaf Samples in a Pilot Study.Foods (Basel, Switzerland) · 2024Article
125 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence, size and importance of bacterial communities on plant leaf surfaces are widely appreciated. However, information is scarce regarding their composition and how it changes along geographical and seasonal scales. We collected 106 samples of field-grown Romaine lettuce from commercial production regions in California and Arizona during the 2009-2010 crop cycle. Total bacterial populations averaged between 10(5) and 10(6) per gram of tissue, whereas counts of culturable bacteria were on average one (summer season) or two (winter season) orders of magnitude lower. Pyrosequencing of 16S rRNA gene amplicons from 88 samples revealed that Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were the most abundantly represented phyla. At the genus level, Pseudomonas, Bacillus, Massilia, Arthrobacter and Pantoea were the most consistently found across samples, suggesting that they form the bacterial 'core' phyllosphere microbiota on lettuce. The foliar presence of Xanthomonas campestris pv. vitians, which is the causal agent of bacterial leaf spot of lettuce, correlated positively with the relative representation of bacteria from the genus Alkanindiges, but negatively with Bacillus, Erwinia and Pantoea. Summer samples showed an overrepresentation of Enterobacteriaceae sequences and culturable coliforms compared with winter samples. The distance between fields or the timing of a dust storm, but not Romaine cultivar, explained differences in bacterial community composition between several of the fields sampled. As one of the largest surveys of leaf surface microbiology, this study offers new insights into the extent and underlying causes of variability in bacterial community composition on plant leaves as a function of time, space and environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.