ArticlePloS one2012
Natural DNA uptake by Escherichia coli.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Efficient natural plasmid transformation ofPNAS nexus · 2024Article
- Detecting Enteric Pathogens in Low-Risk Drinking Water in Dhaka, Bangladesh: An Assessment of the WHO Water Safety Categories.Tropical medicine and infectious disease · 2023Article
- A Brief Review of Plant Cell Transfection, Gene Transcript Expression, and Genotypic Integration for Enhancing Compound Production.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Transmissibility and Persistence of the Plasmid-Borne Mobile Colistin Resistance Gene,Antibiotics (Basel, Switzerland) · 2022Article
- c-di-GMP inhibits LonA-dependent proteolysis of TfoY in Vibrio cholerae.PLoS genetics · 2020Article
- Soft sweep development of resistance in Escherichia coli under fluoroquinolone stress.Journal of microbiology (Seoul, Korea) · 2019Article
- Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.Structure (London, England : 1993) · 2019Article
- Diversification of the type IV filament superfamily into machines for adhesion, protein secretion, DNA uptake, and motility.PLoS biology · 2019Article
- Functional reconstitution of the type IVa pilus assembly system from enterohaemorrhagic Escherichia coli.Molecular microbiology · 2019Article
- Bacterial 'Grounded' Prophages: Hotspots for Genetic Renovation and Innovation.Frontiers in genetics · 2019Article
- Direct observation of end resection by RecBCD during double-stranded DNA break repair in vivo.Nucleic acids research · 2018Article
- Horizontal Plasmid Transfer by Transformation inFrontiers in microbiology · 2018Review
- Evolution of the Natural Transformation Protein, ComEC, in Bacteria.Frontiers in microbiology · 2018Article
- An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species.AMB Express · 2017Article
- Physical shearing imparts biological activity to DNA and ability to transmit itself horizontally across species and kingdom boundaries.BMC molecular biology · 2017Article
- Silently transformable: the many ways bacteria conceal their built-in capacity of genetic exchange.Current genetics · 2017Review
- Evidence for Contemporary Switching of the O-Antigen Gene Cluster between Shiga Toxin-ProducingFrontiers in microbiology · 2017Article
- Using Sex to Cure the Genome.PLoS biology · 2016Article
- Article
- The Extraintestinal Pathogenic Escherichia coli Factor RqlI Constrains the Genotoxic Effects of the RecQ-Like Helicase RqlH.PLoS pathogens · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Escherichia coli has homologues of the competence genes other species use for DNA uptake and processing, but natural competence and transformation have never been detected. Although we previously showed that these genes are induced by the competence regulator Sxy as in other gamma-proteobacteria, no conditions are known that naturally induce sxy expression. We have now tested whether the competence gene homologues encode a functional DNA uptake machinery and whether DNA uptake leads to recombination, by investigating the effects of plasmid-borne sxy expression on natural competence in a wide variety of E. coli strains. High- and low-level sxy expression alone did not induce transformation in any of the strains tested, despite varying the transforming DNA, its concentration, and the incubation conditions used. Direct measurements of uptake of radiolabelled DNA were below the limit of detection, however transformants were readily detected when recombination functions were provided by the lambda Red recombinase. This is the first demonstration that E. coli sxy expression can induce natural DNA uptake and that E. coli's competence genes do encode a functional uptake machinery. However, the amount of transformation cells undergo is limited both by low levels of DNA uptake and by inefficient DNA processing/recombination.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.