ArticleProceedings of the National Academy of Sciences of the United States of America2012
Two distinct overstretched DNA structures revealed by single-molecule thermodynamics measurements.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed.
- Long-lived pauses reveal tunable kinetic barriers during Holliday junction migration.Nucleic acids research · 2026Article
- Local cation-clamping distorts and softens RNA duplex.Communications biology · 2026Article
- ANKLE1 processes chromatin bridges by cleaving mechanically stressed DNA.Nature communications · 2025Article
- The origin of different bending stiffness between double-stranded RNA and DNA revealed by magnetic tweezers and simulations.Nucleic acids research · 2024Article
- Molecular mechanocytometry using tension-activated cell tagging.Nature methods · 2023Article
- Temperature-dependent elastic properties of DNA.Biophysical reports · 2022Article
- Cisplatin fastens chromatin irreversibly even at a high chloride concentration.Nucleic acids research · 2021Article
- Energetics of twisted DNA topologies.Biophysical journal · 2021Review
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- Statistical mechanics of a double-stranded rod model for DNA melting and elasticity.Soft matter · 2020Article
- GC-Content Dependence of Elastic and Overstretching Properties of DNA:RNA Hybrid Duplexes.Biophysical journal · 2020Article
- Dynamic topology of double-stranded telomeric DNA studied by single-molecule manipulation in vitro.Nucleic acids research · 2020Article
- Structural Flexibility of DNA-RNA Hybrid Duplex: Stretching and Twist-Stretch Coupling.Biophysical journal · 2019Article
- Nanomechanics of Diaminopurine-Substituted DNA.Biophysical journal · 2019Article
- The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers.Biophysical journal · 2019Article
- Sequence-Dependent Three Interaction Site Model for Single- and Double-Stranded DNA.Journal of chemical theory and computation · 2018Article
- Hyperstretching DNA.Nature communications · 2017Article
- Freezing shortens the lifetime of DNA molecules under tension.Journal of biological physics · 2017Article
- mDia1 senses both force and torque during F-actin filament polymerization.Nature communications · 2017Article
- Parallel triplex structure formed between stretched single-stranded DNA and homologous duplex DNA.Nucleic acids research · 2017Article
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5 authors.
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Abstract
Double-stranded DNA is a dynamic molecule whose structure can change depending on conditions. While there is consensus in the literature about many structures DNA can have, the state of highly-stretched DNA is still not clear. Several groups have shown that DNA in the torsion-unconstrained B-form undergoes an "overstretching" transition at a stretching force of around 65 pN, which leads to approximately 1.7-fold elongation of the DNA contour length. Recent experiments have revealed that two distinct structural transitions are involved in the overstretching process: (i) a hysteretic "peeling" off one strand from its complementary strand, and (ii) a nonhysteretic transition that leads to an undetermined DNA structure. We report the first simultaneous determination of the entropy (ΔS) and enthalpy changes (ΔH) pertaining to these respective transitions. For the hysteretic peeling transition, we determined ΔS ∼ 20 cal/(K.mol) and ΔH ∼ 7 kcal/mol. In the case of the nonhysteretic transition, ΔS ∼ -3 cal/(K.mol) and ΔH ∼ 1 kcal/mol. Furthermore, the response of the transition force to salt concentration implies that the two DNA strands are spatially separated after the hysteretic peeling transition. In contrast, the corresponding response after the nonhysteretic transition indicated that the strands remained in close proximity. The selection between the two transitions depends on DNA base-pair stability, and it can be illustrated by a multidimensional phase diagram. Our results provide important insights into the thermodynamics of DNA overstretching and conformational structures of overstretched DNA that may play an important role in vivo.
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