ArticlePlanta2012
A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Selective degradation processes in plants are not limited to autophagy.Plant cell reports · 2026Review
- Physiological and transcriptomic assessment of Arabidopsis identifies two distinct gravity signaling systems.Plant physiology · 2026Article
- Gravity beyond starch: molecular evidence for a parallel gravity sensing mechanism.Plant physiology · 2026Article
- Review
- Phenylmercury stress induces root tip swelling through auxin homeostasis disruption.Plant molecular biology · 2024Article
- Role of Abscisic Acid, Reactive Oxygen Species, and CaPlants (Basel, Switzerland) · 2024Review
- Dynamic organelle changes and autophagic processes in lily pollen germination.Botanical studies · 2024Article
- The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (Plants (Basel, Switzerland) · 2023Article
- Functional specialization of chloroplast vesiculation (Frontiers in plant science · 2023Article
- Leveraging orthology within maize and Arabidopsis QTL to identify genes affecting natural variation in gravitropism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Root osmotic sensing from local perception to systemic responses.Stress biology · 2022Review
- Autophagy promotes organelle clearance and organized cell separation of living root cap cells in Arabidopsis thaliana.Development (Cambridge, England) · 2022Article
- Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism.International journal of molecular sciences · 2022Article
- Experimental Manipulation of Pectin Architecture in the Cell Wall of the Unicellular Charophyte,Frontiers in plant science · 2020Article
- Molecular mechanisms mediating root hydrotropism: what we have observed since the rediscovery of hydrotropism.Journal of plant research · 2020Review
- Selective Elimination of Membrane-Damaged Chloroplasts via Microautophagy.Plant physiology · 2018Article
- Entire Photodamaged Chloroplasts Are Transported to the Central Vacuole by Autophagy.The Plant cell · 2017Article
- Reactive Oxygen Species Tune Root Tropic Responses.Plant physiology · 2016Article
- Growing Out of Stress: The Role of Cell- and Organ-Scale Growth Control in Plant Water-Stress Responses.The Plant cell · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seedling roots display not only gravitropism but also hydrotropism, and the two tropisms interfere with one another. In Arabidopsis (Arabidopsis thaliana) roots, amyloplasts in columella cells are rapidly degraded during the hydrotropic response. Degradation of amyloplasts involved in gravisensing enhances the hydrotropic response by reducing the gravitropic response. However, the mechanism by which amyloplasts are degraded in hydrotropically responding roots remains unknown. In this study, the mechanistic aspects of the degradation of amyloplasts in columella cells during hydrotropic response were investigated by analyzing organellar morphology, cell polarity and changes in gene expression. The results showed that hydrotropic stimulation or systemic water stress caused dramatic changes in organellar form and positioning in columella cells. Specifically, the columella cells of hydrotropically responding or water-stressed roots lost polarity in the distribution of the endoplasmic reticulum (ER), and showed accelerated vacuolization and nuclear movement. Analysis of ER-localized GFP showed that ER redistributed around the developed vacuoles. Cells often showed decomposing amyloplasts in autophagosome-like structures. Both hydrotropic stimulation and water stress upregulated the expression of AtATG18a, which is required for autophagosome formation. Furthermore, analysis with GFP-AtATG8a revealed that both hydrotropic stimulation and water stress induced the formation of autophagosomes in the columella cells. In addition, expression of plastid marker, pt-GFP, in the columella cells dramatically decreased in response to both hydrotropic stimulation and water stress, but its decrease was much less in the autophagy mutant atg5. These results suggest that hydrotropic stimulation confers water stress in the roots, which triggers an autophagic response responsible for the degradation of amyloplasts in columella cells of Arabidopsis roots.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.