ArticleInvestigative ophthalmology & visual science2012
Two-photon immunofluorescence characterization of the trabecular meshwork in situ.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Visualization of porcine and human aqueous humor outflow tract anatomies with transparency enhancement.Japanese journal of ophthalmology · 2025Article
- Advancements in high-resolution imaging of the iridocorneal angle.Frontiers in ophthalmology · 2023Review
- Two-photon excitation fluorescence in ophthalmology: safety and improved imaging for functional diagnostics.Frontiers in medicine · 2023Review
- Valve-Like Outflow System Behavior With Motion Slowing in Glaucoma Eyes: Findings Using a Minimally Invasive Glaucoma Surgery-MIGS-Like Platform and Optical Coherence Tomography Imaging.Frontiers in medicine · 2022Article
- Aqueous outflow regulation - 21st century concepts.Progress in retinal and eye research · 2021Review
- Crosstalk between transforming growth factor β-2 and Autotaxin in trabecular meshwork and different subtypes of glaucoma.Journal of biomedical science · 2021Article
- Ex-vivo cultured human corneoscleral segment model to study the effects of glaucoma factors on trabecular meshwork.PloS one · 2020Article
- High-Resolution, Adaptive Optics Imaging of the Human Trabecular Meshwork In Vivo.Translational vision science & technology · 2019Article
- In vivo two-photon microscopy of the human eye.Scientific reports · 2019Article
- Ultrastructural variability of the juxtacanalicular tissue along the inner wall of Schlemm's canal.Molecular vision · 2019Article
- Interleukin-6-mediated trans-signaling inhibits transforming growth factor-β signaling in trabecular meshwork cells.The Journal of biological chemistry · 2018Article
- Deep tissue analysis of distal aqueous drainage structures and contractile features.Scientific reports · 2017Article
- Toward in vivo two-photon analysis of mouse aqueous outflow structure and function.Experimental eye research · 2017Review
- Aqueous Humor Outflow Structure and Function Imaging At the Bench and Bedside: A Review.Journal of clinical & experimental ophthalmology · 2016Article
- Dose- and time-dependent effects of actomyosin inhibition on live mouse outflow resistance and aqueous drainage tissues.Scientific reports · 2016Article
- Tissue-based multiphoton analysis of actomyosin and structural responses in human trabecular meshwork.Scientific reports · 2016Article
- Optimizing two-photon multiple fluorophore imaging of the human trabecular meshwork.Molecular vision · 2016Article
- Observing live actin in the human trabecular meshwork.Clinical & experimental ophthalmology · 2014Article
- Sources of structural autofluorescence in the human trabecular meshwork.Investigative ophthalmology & visual science · 2013Article
- The Juxtacanalicular Region of Ocular Trabecular Meshwork: A Tissue with a Unique Extracellular Matrix and Specialized Function.Journal of ocular biology · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo develop an in situ model to study biological responses and glaucoma pathology in the human trabecular meshwork (TM). Characteristic TM cell- and glaucoma-associated markers were localized in situ in relation to the tissue's autofluorescent structural extracellular matrix (ECM) by two-photon excitation fluorescence optical sectioning (TPEF).
methodsHuman donor corneoscleral (CS) tissue containing the intact aqueous drainage tract was incubated with dexamethasone (Dex) or TGF-β1, and immunostained for epifluorescence (EF) microscopy with antibodies to myocilin and alpha smooth muscle (α-SMA). Separate specimens were labeled for Type-IV collagen and fibronectin. Nuclei were stained with Hoechst 33342. Multimodal TPEF was used to visualize EF, intravital dyes, and autofluorescence (AF) in situ. Three-dimensional (3D) localization of fluorescence within the TM was analyzed using reconstruction software.
resultsAutofluorescent beams, perforated sheets, and fibers, consistent with the uveal (UV), CS, and juxtacanalicular (JCT) meshwork, respectively, were captured at different depths of the TM. Type-IV collagen EF distinctly outlined the AF beams in a location consistent with basement membrane. Fibronectin EF showed a diffuse reticular pattern throughout the TM. TGF-β1-induced α-SMA expression, which was distributed perinuclearly in cells among autofluorescent structures. Dex-induced myocilin expression had both cytosolic and extracellular distributions.
conclusionsThe authors have localized markers that are characteristic of TM cells and relevant to glaucoma pathogenesis in situ using multimodal TPEF without conventional histological embedding and sectioning. Protein expression was inducible in situ and could be analyzed with respect to cells and the ECM within the 3D environment of the human TM.
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