ArticleInternational journal of obesity (2005)2013
Determination of the obesity-associated gene variants within the entire FTO gene by ultra-deep targeted sequencing in obese and lean children.
Article in International journal of obesity (2005), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- FTO gene variant and risk of overweight and obesity among children and adolescents: a systematic review and meta-analysis.PloS one · 2013Pooled it
- An evaluation of age-varying genetic effects underlying body-mass index and blood pressure in the UK Biobank.PLoS genetics · 2026Article
- Next-Generation Sequencing of a Large Gene Panel for Outcome Prediction of Bariatric Surgery in Patients with Severe Obesity.Journal of clinical medicine · 2022Article
- Article
- The association between sarcopenia susceptibility and polymorphisms of FTO, ACVR2B, and IRS1 in Tibetans.Molecular genetics & genomic medicine · 2021Article
- Genome Database of the Latvian Population (LGDB): Design, Goals, and Primary Results.Journal of epidemiology · 2018Article
- Disruption of Accumbens and Thalamic White Matter Connectivity Revealed by Diffusion Tensor Tractography in Young Men with Genetic Risk for Obesity.Frontiers in human neuroscience · 2018Article
- The association of insertions/deletions (INDELs) and variable number tandem repeats (VNTRs) with obesity and its related traits and complications.Journal of physiological anthropology · 2017Review
- Resting-State Brain and the FTO Obesity Risk Allele: Default Mode, Sensorimotor, and Salience Network Connectivity Underlying Different Somatosensory Integration and Reward Processing between Genotypes.Frontiers in human neuroscience · 2016Article
- Determination of obesity associated gene variants related to TMEM18 through ultra-deep targeted re-sequencing in a case-control cohort for pediatric obesity.Genetics research · 2015Article
- Current review of genetics of human obesity: from molecular mechanisms to an evolutionary perspective.Molecular genetics and genomics : MGG · 2015Review
- Obesity and genomics: role of technology in unraveling the complex genetic architecture of obesity.Human genetics · 2015Review
- The 'Fat Mass and Obesity Related' (FTO) gene: Mechanisms of Impact on Obesity and Energy Balance.Current obesity reports · 2015Review
- Fat mass and obesity-associated gene rs11642015 polymorphism is significantly associated with prediabetes and type 2 diabetes subsequent to adjustment for body mass index.Biomedical reports · 2014Article
- Sequence variation in TMEM18 in association with body mass index: Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Consortium Targeted Sequencing Study.Circulation. Cardiovascular genetics · 2014Article
- The role of common and rare MC4R variants and FTO polymorphisms in extreme form of obesity.Molecular biology reports · 2014Article
- A 680 kb duplication at the FTO locus in a kindred with obesity and a distinct body fat distribution.European journal of human genetics : EJHG · 2013Article
- Lessons learned from implementing a national infrastructure in Sweden for storage and analysis of next-generation sequencing data.GigaScience · 2013Article
- Article
- A variant in FTO shows association with melanoma risk not due to BMI.Nature genetics · 2013Article
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Fat mass and obesity-associated gene (FTO) was the first gene reliably associated with body mass index in genome-wide association studies on a population level. At present, the genetic variations within the FTO gene are still the common variants that have the largest influence on body mass index.
methodsIn the current study, we amplified the entire FTO gene, in total 412 Kbp, in over 200 long-range PCR fragments from each individual, from 524 severely obese and 527 lean Swedish children, and sequenced the products as two DNA pools using massive parallel sequencing (SOLiD).
resultsThe sequencing achieved very high coverage (median 18 000 reads) and we detected and estimated allele frequencies for 705 single nucleotide polymorphisms (SNPs) (19 novel) and 40 indels (24 novel) using a sophisticated statistical approach to remove false-positive SNPs. We identified 19 obesity-associated SNPs within intron one of the FTO gene, and validated our findings with genotyping. Ten of the validated obesity-associated SNPs have a stronger obesity association (P<0.007) than the commonly studied rs9939609 SNP (P<0.012).
conclusionsThis study provides a comprehensive obesity-associated variation map of FTO, identifies novel lead SNPs and evaluates putative causative variants. We conclude that intron one is the only region within the FTO gene associated with obesity, and finally, we establish next generation sequencing of pooled DNA as a powerful method to investigate genetic association with complex diseases and traits.
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