ArticleAnalytical chemistry2012
ToF-SIMS depth profiling of cells: z-correction, 3D imaging, and sputter rate of individual NIH/3T3 fibroblasts.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Article
- Depth Correction of TOF-SIMS Depth Profiling Images Using the Total Ion Count Images.Biomolecules · 2025Article
- Exploring Single-Probe Single-Cell Mass Spectrometry: Current Trends and Future Directions.Analytical chemistry · 2025Review
- Rational Design of an In-Situ Polymer-Inorganic Hybrid Solid Electrolyte Interphase for Realising Stable Zn Metal Anode under Harsh Conditions.Angewandte Chemie (International ed. in English) · 2024Article
- Single Cell mass spectrometry: Towards quantification of small molecules in individual cells.Trends in analytical chemistry : TRAC · 2024Article
- Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology.Nature communications · 2023Article
- Advancements in ToF-SIMS imaging for life sciences.Frontiers in chemistry · 2023Review
- Role of lipidomics in assessing the functional lipid composition in breast milk.Frontiers in nutrition · 2022Review
- Depth correction of 3D NanoSIMS images using secondary electron pixel intensities.Biointerphases · 2021Article
- Subcellular localization of biomolecules and drug distribution by high-definition ion beam imaging.Nature communications · 2021Article
- Developments and Ongoing Challenges for Analysis of Surface-Bound Proteins.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Article
- Nanoscopic subcellular imaging enabled by ion beam tomography.Nature communications · 2021Article
- Spatially resolved 3D metabolomic profiling in tissues.Science advances · 2021Article
- Investigation of Inter- and Intratumoral Heterogeneity of Glioblastoma Using TOF-SIMS.Molecular & cellular proteomics : MCP · 2020Article
- Time-of-flight secondary ion mass spectrometry three-dimensional imaging of surface modifications in poly(caprolactone) scaffold pores.Journal of biomedical materials research. Part A · 2019Article
- Tutorial on lipidomics.Analytica chimica acta · 2019Review
- Exploring the Fundamental Structures of Life: Non-Targeted, Chemical Analysis of Single Cells and Subcellular Structures.Angewandte Chemie (International ed. in English) · 2019Review
- Three Dimensional Secondary Ion Mass Spectrometry Imaging (3D-SIMS) ofJournal of analytical atomic spectrometry · 2019Article
- Internal Energy Distribution of Secondary Ions Under Argon and Bismuth Cluster Bombardments: "Soft" Versus "Hard" Desorption-Ionization Process.Journal of the American Society for Mass Spectrometry · 2019Article
- Correlative microscopy of freeze-dried cells and studies on intracellular calcium stores with imaging secondary ion mass spectrometry (SIMS).Journal of analytical atomic spectrometry · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Proper display of three-dimensional time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging data of complex, nonflat samples requires a correction of the data in the z-direction. Inaccuracies in displaying three-dimensional ToF-SIMS data arise from projecting data from a nonflat surface onto a 2D image plane, as well as possible variations in the sputter rate of the sample being probed. The current study builds on previous studies by creating software written in Matlab, the ZCorrectorGUI (available at http://mvsa.nb.uw.edu/), to apply the z-correction to entire 3D data sets. Three-dimensional image data sets were acquired from NIH/3T3 fibroblasts by collecting ToF-SIMS images, using a dual beam approach (25 keV Bi(3)(+) for analysis cycles and 20 keV C(60)(2+) for sputter cycles). The entire data cube was then corrected by using the new ZCorrectorGUI software, producing accurate chemical information from single cells in 3D. For the first time, a three-dimensional corrected view of a lipid-rich subcellular region, possibly the nuclear membrane, is presented. Additionally, the key assumption of a constant sputter rate throughout the data acquisition was tested by using ToF-SIMS and atomic force microscopy (AFM) analysis of the same cells. For the dried NIH/3T3 fibroblasts examined in this study, the sputter rate was found to not change appreciably in x, y, or z, and the cellular material was sputtered at a rate of approximately 10 nm per 1.25 × 10(13) ions C(60)(2+)/cm(2).
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