ArticlePloS one2012
Inverse fusion PCR cloning.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- IVA Prime: automated primer design for in vivo assembly cloning.Nucleic acids research · 2025Article
- Circular PCR as an efficient and precise umbrella of methods for the generation of circular dsDNA with staggered nicks: Mechanism and types.Biology methods & protocols · 2024Article
- Accelerated Long-Fragment Circular PCR for Genetic Manipulation of Plant Viruses in Unveiling Functional Genomics.Viruses · 2023Article
- A Simple and Affordable Method to Create Nonsense Mutation Clones of p53 for Studying the Premature Termination Codon Readthrough Activity of PTC124.Biomedicines · 2023Article
- Structural Modeling of the Treponema pallidum Outer Membrane Protein Repertoire: a Road Map for Deconvolution of Syphilis Pathogenesis and Development of a Syphilis Vaccine.Journal of bacteriology · 2021Article
- Alternative Seamless Cloning Strategies in Fusing Gene Fragments Based on Overlap-PCR.Molecular biotechnology · 2021Article
- Article
- Intra-Molecular Homologous Recombination of Scarless Plasmid.International journal of molecular sciences · 2020Article
- Mega primer-mediated molecular cloning strategy for chimaeragenesis and long DNA fragment insertion.Bioscience reports · 2017Article
- OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments.Scientific reports · 2017Article
- IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly.Scientific reports · 2016Article
- QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids.Journal of biological engineering · 2015Article
- Article
- Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V.BMC biotechnology · 2013Article
- Ultra-low background DNA cloning system.PloS one · 2013Article
- Generating in vivo cloning vectors for parallel cloning of large gene clusters by homologous recombination.PloS one · 2013Article
- Plasmids for increased efficiency of vector construction and genetic engineering in filamentous fungi.Fungal genetics and biology : FG & BArticle
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inverse fusion PCR cloning (IFPC) is an easy, PCR based three-step cloning method that allows the seamless and directional insertion of PCR products into virtually all plasmids, this with a free choice of the insertion site. The PCR-derived inserts contain a vector-complementary 5'-end that allows a fusion with the vector by an overlap extension PCR, and the resulting amplified insert-vector fusions are then circularized by ligation prior transformation. A minimal amount of starting material is needed and experimental steps are reduced. Untreated circular plasmid, or alternatively bacteria containing the plasmid, can be used as templates for the insertion, and clean-up of the insert fragment is not urgently required. The whole cloning procedure can be performed within a minimal hands-on time and results in the generation of hundreds to ten-thousands of positive colonies, with a minimal background.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.