ArticleWorld journal of gastroenterology2012
Overexpression of Dickkopf-3 induces apoptosis through mitochondrial pathway in human colon cancer.
Article in World journal of gastroenterology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- DKK3 promotes renal fibrosis by increasing MFF-mediated mitochondrial dysfunction in Wnt/β-catenin pathway-dependent manner.Renal failure · 2024Article
- Deep Sc-RNA sequencing decoding the molecular dynamic architecture of the human retina.Science China. Life sciences · 2023Article
- Article
- miR-1290 contributes to oncogenesis and angiogenesis via targeting of THBS1, DKK3 and, SCAI.BioImpacts : BI · 2022Article
- MiR-425 Promotes Migration and Invasion in Bladder Cancer by Targeting Dickkopf 3.Journal of Cancer · 2020Article
- Loss of FFAR2 promotes colon cancer by epigenetic dysregulation of inflammation suppressors.International journal of cancer · 2018Article
- Dkk-3 as a potential biomarker for diagnosis and prognosis of colorectal cancer.Medical journal of the Islamic Republic of Iran · 2018Article
- Dickkopf-3 Causes Neuroprotection by Inducing Vascular Endothelial Growth Factor.Frontiers in cellular neuroscience · 2018Article
- Dickkopf-3 Ablation Attenuates the Development of Atherosclerosis in ApoE-Deficient Mice.Journal of the American Heart Association · 2017Article
- A Novel Role of Dickkopf-Related Protein 3 in Macropinocytosis in Human Bladder Cancer T24 Cells.International journal of molecular sciences · 2016Article
- Dkk3 prevents familial dilated cardiomyopathy development through Wnt pathway.Laboratory investigation; a journal of technical methods and pathology · 2016Article
- DKK3 blocked translocation of β-catenin/EMT induced by hypoxia and improved gemcitabine therapeutic effect in pancreatic cancer Bxpc-3 cell.Journal of cellular and molecular medicine · 2015Article
- Identification of secreted proteins that reflect autophagy dynamics within tumor cells.Autophagy · 2015Article
- Role of Intracellular and Extracellular MicroRNA-92a in Colorectal Cancer.Translational oncology · 2013Article
- Cidofovir selectivity is based on the different response of normal and cancer cells to DNA damage.BMC medical genomics · 2013Article
- Expression profile of altered long non-coding RNAs in patients with HBV-associated hepatocellular carcinoma.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2013Article
- Dkk-3, a secreted wnt antagonist, suppresses tumorigenic potential and pulmonary metastasis in osteosarcoma.Sarcoma · 2013Article
- p53, SKP2, and DKK3 as MYCN Target Genes and Their Potential Therapeutic Significance.Frontiers in oncology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the mechanisms of the biological roles of Dickkopf-3 (Dkk-3) in cell invasion, survival and apoptosis in colon cancer cells.
methodsThree human colon cancer cell lines, i.e., HT-29, LoVo and SW480, were used. Overexpression of Dkk-3 induced by pEGFP-N1-Dkk-3-GFP plasmid in LoVo cells was performed using Lipofectamine 2000 reagent. Reverse transcription polymerase chain reaction and Western blotting were performed to determine the mRNA and protein expression levels of Dkk-3, respectively. Cell proliferation assay, cell cycle analysis, hoechst 33258 assay and Matrigel invasion assay were performed on Dkk-3 overexpressing transfectants.
resultsThe mRNA and protein expressions of Dkk-3 in HT-29 (mRNA: 0.06 ± 0.02, protein: 0.06 ± 0.01) and LoVo (mRNA: 0.07 ± 0.02, protein: 0.07 ± 0.02) cells were significantly lower than that in SW480 cells (mRNA: 0.92 ± 0.04, protein: 0.69 ± 0.13; all P < 0.05), and the greatest levels of invasiveness was in LoVo cells. Dkk-3 overexpression inhibited the proliferation and invasion of LoVo cells and induced cell cycle arrest at G(0)/G(1) phase and subsequent apoptosis, as indicated by increased chromatin condensation and fragments, upregulated Bax and cytochrome c protein, downregulated survivin and Bcl-2 protein, and the activation of caspase-3 and caspase-9. Furthermore, Dkk-3 overexpression reduced the accumulation of cytosolic fraction of β-catenin.
conclusionDkk-3 overexpression induced apoptosis in human colon cancer possibly through the mitochondrial pathway. Dkk-3 may be involved in the Wnt/β-catenin signaling pathways in colon cancer.
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