Evidence map›Paper›PMID 22529214›Full record

ArticleEndocrinology2012

Dynamic changes in cervical glycosaminoglycan composition during normal pregnancy and preterm birth.

Yucel Akgul, Roxane Holt, Mark Mummert, Ann Word, Mala Mahendroo

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed, 2 pooled it
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 2 syntheses or guidelines pooled it, 99 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Observational
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
  18. Article
  19. Review
  20. Article

4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yucel AkgulDepartment of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas Texas 75390-9032, USA.
Roxane Holt
Mark Mummert
Ann Word
Mala Mahendroo
Southwestern Medical Center · USUniversity of North Texas · US

Funding

TRANSCRIPTIONAL REGULATION OF CERVICAL COMPETENCE DURING PREGNANCYP01HD011149 · NICHD · UT SOUTHWESTERN MEDICAL CENTER · PI MENDELSON, CAROLE R · 1998 to 2015
$17.7M
NICHD NIH HHS 5-PO1-HD011149NICHD NIH HHS P01 HD011149
6 · The paper itself

Abstract

Glycosaminoglycans (GAG) have diverse functions that regulate macromolecular assembly in the extracellular matrix. During pregnancy, the rigid cervix transforms to a pliable structure to allow birth. Quantitative assessment of cervical GAG is a prerequisite to identify GAG functions in term and preterm birth. In the current study, total GAG levels increased at term, yet the abundance, chain length, and sulfation levels of sulfated GAG remained constant. The increase in total GAG resulted exclusively from an increase in hyaluronan (HA). HA can form large structures that promote increased viscosity, hydration, and matrix disorganization as well as small structures that have roles in inflammation. HA levels increased from 19% of total GAG in early pregnancy to 71% at term. Activity of the HA-metabolizing enzyme, hyaluronidase, increased in labor, resulting in metabolism of large to small HA. Similar to mice, HA transitions from high to low molecular weight in term human cervix. Mouse preterm models were also characterized by an increase in HA resulting from differential expression of the HA synthase (Has) genes, with increased Has1 in preterm in contrast to Has2 induction at term. The Has2 gene but not Has1 is regulated in part by estrogen. These studies identify a shift in sulfated GAG dominance in the early pregnant cervix to HA dominance in term and preterm ripening. Increased HA synthesis along with hyaluronidase-induced changes in HA size in mice and women suggest diverse contributions of HA to macromolecular changes in the extracellular matrix, resulting in loss of tensile strength during parturition.

Indexed as

AnimalsCervix UteriFemaleGene Expression ProfilingGene Expression Regulation, EnzymologicGlucuronosyltransferaseGlycosaminoglycansHumansHyaluronan SynthasesHyaluronic AcidMiceMice, Inbred C57BLParturitionPregnancyPremature BirthReceptors, EstrogenGlucuronosyltransferaseGlycosaminoglycansHAS1 protein, humanHAS2 protein, humanHas2 protein, mouseHyaluronan SynthasesHyaluronic AcidReceptors, Estrogen

Identifiers

PMID22529214
PMCPMC3380303
OpenAlexW2004588162

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.