Evidence map›Paper›PMID 22528100›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2012

Study of amyloids using yeast.

Reed B Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, Herman K Edskes

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Heat shock protein 104 (Hsp104)-mediated curing of [The Journal of biological chemistry · 2017
    Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Reed B WicknerLaboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. wickner@helix.nih.gov
Dmitry Kryndushkin
Frank Shewmaker
Ryan McGlinchey
Herman K Edskes
National Institute of Diabetes and Digestive and Kidney Diseases · USNational Institutes of Health · USUniformed Services University of the Health Sciences · US

Funding

Prions of Yeast and Anti-Prion SystemsZIADK024950 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI WICKNER, REED B. · 2009 to 2025
$25.2M
Biological Roles of Yeast PrionsZ01DK024950 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI WICKNER, REED B. · 2007 to 2008
$999k
Intramural NIH HHS Z01 DK024950Intramural NIH HHS ZIA DK024950
6 · The paper itself

Abstract

We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.

Indexed as

AmyloidAspartic AcidBiological TransportChromatography, LiquidElectrophoresis, Polyacrylamide GelEndopeptidase KGene FusionGenetic VectorsLuminescent ProteinsMagnetic Resonance SpectroscopyMass SpectrometryMicroscopy, Electron, TransmissionPhenotypePrionsProtein MultimerizationProtein Structure, SecondaryAmyloidAspartic AcidEndopeptidase KLuminescent ProteinsPrionsSaccharomyces cerevisiae ProteinsUracilureidosuccinic acid

Identifiers

PMID22528100
PMCPMC3479635
OpenAlexW175806149

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.