ArticleJournal of assisted reproduction and genetics2012
Triploid and diploid embryonic stem cell lines derived from tripronuclear human zygotes.
Article in Journal of assisted reproduction and genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Genomic stability of human pluripotent stem cells: advances in research and screening criteria.Stem cells translational medicine · 2026Review
- Scoping review on the embryonic developmental potential of tripronuclear zygotes and their use for clinical and research purposes.Journal of assisted reproduction and genetics · 2025Article
- Generation, genomic characterization, and differentiation of triploid human embryonic stem cells.Stem cell reports · 2023Article
- Derivation of human triploid trophoblast stem cells.Journal of assisted reproduction and genetics · 2022Article
- Mesenchymal Stem/Stromal-Like Cells from Diploid and Triploid Human Embryonic Stem Cells Display Different Gene Expression Profiles.Iranian biomedical journal · 2021Article
- Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells.The Journal of reproduction and development · 2016Article
- Developmental potential of clinically discarded human embryos and associated chromosomal analysis.Scientific reports · 2016Article
- The parental origin correlates with the karyotype of human embryos developing from tripronuclear zygotes.Clinical and experimental reproductive medicine · 2015Article
- Improved efficiency of microsurgical enucleated tripronuclear zygotes development and embryonic stem cell derivation by supplementing epidermal growth factor, brain-derived neurotrophic factor, and insulin-like growth factor-1.Stem cells and development · 2014Article
- Diploid, but not haploid, human embryonic stem cells can be derived from microsurgically repaired tripronuclear human zygotes.Cell cycle (Georgetown, Tex.) · 2013Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeHuman embryonic stem cells (hESCs) are self-renewing, pluripotent cells that are valuable research tools and hold promise for use in regenerative medicine. The need for new hESC lines motivated our attempts to find a new resource for the derivation of hESC lines. The aim of this work was to establish more hESC lines from abnormal fertilized zygotes and to meet the emerging requirements for their use in cell replacement therapies, disease modeling, and basic research.
methodsA total of 130 tripronuclear human zygotes was collected 18-20 h post-insemination and cultured in a modified culture medium. The inner cell mass of 12 blastocysts were isolated by a mechanical method in order to establish embryonic stem cell lines.
resultsWe established four hESC lines derived from 130 trinuclear zygotes, one of which was triploid and the others were diploid. The efficiency of deriving hESC lines is 3.08 %. The ratio of deriving triploid and diploid hESC lines is 1:3. All of these hESC lines exhibited similar markers of undifferentiated hESCs and had the typical morphology of hESCs, a capacity for long-term proliferation, and pluripotent differentiation potential both in vivo and in vitro.
conclusionsThese abnormal zygotes, which otherwise would have been discarded, can serve as an alternative source for normal euploid hESC lines.
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