ArticlePlant molecular biology2012
Deep EST profiling of developing fenugreek endosperm to investigate galactomannan biosynthesis and its regulation.
Article in Plant molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 35 citations in OpenAlex.
- Co-expression of AkCSLA3 and AkMSR1 correlates with altered free monosaccharide pools in Pichia pastoris.BMC plant biology · 2026Article
- Transcriptomic reveals key genes and regulatory pathways in galactomannan biosynthesis inFrontiers in plant science · 2025Article
- De novo sequencing allows genome-wide identification of genes involved in galactomannan synthesis in locust bean (Ceratonia siliqua).DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
- Glucomannan inGenes · 2022Review
- Transcriptome analysis associated with polysaccharide synthesis and their antioxidant activity inPeerJ · 2021Article
- Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.BMC plant biology · 2019Article
- Article
- Mechanistic insights from plant heteromannan synthesis in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A Review: The Bioactivities and Pharmacological Applications of Polygonatum sibiricum polysaccharides.Molecules (Basel, Switzerland) · 2018Review
- De Novo Assembly and Analysis of Polygonatum sibiricum Transcriptome and Identification of Genes Involved in Polysaccharide Biosynthesis.International journal of molecular sciences · 2017Article
- MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage.Plant physiology · 2015Article
- Identification of genes involved in biosynthesis of mannan polysaccharides in Dendrobium officinale by RNA-seq analysis.Plant molecular biology · 2015Article
- Transcriptome sequencing of Codonopsis pilosula and identification of candidate genes involved in polysaccharide biosynthesis.PloS one · 2015Article
- Transcription factors that directly regulate the expression of CSLA9 encoding mannan synthase in Arabidopsis thaliana.Plant molecular biology · 2014Article
- Hemicellulose biosynthesis.Planta · 2013Review
- Identification of an additional protein involved in mannan biosynthesis.The Plant journal : for cell and molecular biology · 2013Article
- Discovery of diversity in xylan biosynthetic genes by transcriptional profiling of a heteroxylan containing mucilaginous tissue.Frontiers in plant science · 2013Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Galactomannans are hemicellulosic polysaccharides composed of a (1 → 4)-linked β-D-mannan backbone substituted with single-unit (1 → 6)-α-linked D-galactosyl residues. Developing fenugreek (Trigonella foenum-graecum) seeds are known to accumulate large quantities of galactomannans in the endosperm, and were thus used here as a model system to better understand galactomannan biosynthesis and its regulation. We first verified the specific deposition of galactomannans in developing endosperms and determined that active accumulation occurred from 25 to 38 days post anthesis (DPA) under our growth conditions. We then examined the expression levels during seed development of ManS and GMGT, two genes encoding backbone and side chain synthetic enzymes. Based on transcript accumulation dynamics for ManS and GMGT, cDNA libraries were constructed using RNA isolated from endosperms at four ages corresponding to before, at the beginning of, and during active galactomannan deposition. DNA from these libraries was sequenced using the 454 sequencing technology to yield a total of 1.5 million expressed sequence tags (ESTs). Through analysis of the EST profiling data, we identified genes known to be involved in galactomannan biosynthesis, as well as new genes that may be involved in this process, and proposed a model for the flow of carbon from sucrose to galactomannans. Measurement of in vitro ManS and GMGT activities and analysis of sugar phosphate and nucleotide sugar levels in the endosperms of developing fenugreek seeds provided data consistent with this model. In vitro enzymatic assays also revealed that the ManS enzyme from fenugreek endosperm preferentially used GDP-mannose as the substrate for the backbone synthesis.
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