ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2012
Novel diagnosis of fungal endophthalmitis by broad-range real-time PCR detection of fungal 28S ribosomal DNA.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Understanding the science of fungal endophthalmitis - AIOS 2021 Sengamedu Srinivas Badrinath Endowment Lecture.Indian journal of ophthalmology · 2022Trial
- Comprehensive polymerase chain reaction assay for detection of pathogenic DNA in lymphoproliferative disorders of the ocular adnexa.Scientific reports · 2016Trial
- Enhanced Detection of Bacterial Ocular Pathogens: A Comparative Study of Broad-Range Real-Time PCR and Conventional Culture Methods.Diagnostics (Basel, Switzerland) · 2025Article
- Universal PCR for bacteria, mycobacteria, and fungi: a 10-year retrospective review of clinical indications and patient outcomes.Journal of clinical microbiology · 2023Article
- An update on immunological and molecular tests and their impact in infectious uveitis.Frontiers in ophthalmology · 2023Review
- Epstein-Barr viral corneal stromal keratitis occurring during rheumatoid arthritis treatment: a case report.BMC ophthalmology · 2022Article
- Clinical features of uveitis in elderly patients in central Tokyo (2013-2018).International ophthalmology · 2021Article
- Practical use of multiplex and broad-range PCR in ophthalmology.Japanese journal of ophthalmology · 2021Review
- Exogenous Fungal Endophthalmitis: Clues toMicroorganisms · 2020Review
- The cereus matter of Bacillus endophthalmitis.Experimental eye research · 2020Review
- Bilateral granulomatous panuveitis in two patients with T-cell type of chronic active Epstein-Barr virus infection.BMC ophthalmology · 2019Article
- Epidemiology of uveitis (2013-2015) and changes in the patterns of uveitis (2004-2015) in the central Tokyo area: a retrospective study.BMC ophthalmology · 2018Article
- Chronic postcataract endophthalmitis caused byInternational medical case reports journal · 2018Article
- Assessment of diagnostic and therapeutic vitrectomy for vitreous opacity associated with uveitis with various etiologies.Medicine · 2018Observational
- Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution.Scientific reports · 2017Article
- Uveitis incidence in Jichi Medical University Hospital, Japan, during 2011-2015.Clinical ophthalmology (Auckland, N.Z.) · 2017Article
- A new era of uveitis: impact of polymerase chain reaction in intraocular inflammatory diseases.Japanese journal of ophthalmology · 2017Review
- Emerging techniques for pathogen discovery in endophthalmitis.Current opinion in ophthalmology · 2015Review
- Endophthalmitis: state of the art.Clinical ophthalmology (Auckland, N.Z.) · 2015Review
- Bilateral endogenous Candida albicans subretinal abscess with suspected mixed bacterial infection.Clinical ophthalmology (Auckland, N.Z.) · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo detect the fungal genome in the ocular fluids of patients with fungal endophthalmitis by using a novel broad-range polymerase chain reaction (PCR) system.
methodsAfter informed consent was obtained, ocular fluid samples (aqueous humor or vitreous fluids) were collected from 497 patients (76 patients with infectious endophthalmitis including clinically suspected bacterial and fungal endophthalmitis and 421 patients with infectious or non-infectious uveitis). Forty ocular samples from non-infectious patients without ocular inflammation were collected as controls. Fungal ribosomal DNA (28 S rDNA) was measured by a quantitative real-time PCR assay.
resultsFungal 28 S rDNA of the major fungal species, such as Candida, Aspergillus, and Cryptococcus, were detected by novel broad-range real-time PCR examination (>10(1) copies/ml). Fungal 28 S rDNA was detected in the ocular fluids of 11 patients with endophthalmitis or uveitis (11/497, 2.2%). All 11 positive samples were detected in the infectious endophthalmitis patients (11/76, 14.5%). These PCR-positive ocular fluids had high copy numbers of fungal 28 S rDNA (range, 1.7 × 10(3) to 7.9 × 10(6) copies/ml), which indicated the presence of fungal infection. Of the 11 patients who were PCR positive, further examinations led to a diagnosis of fungal endophthalmitis in ten patients. The fungal 28 S rDNA was detected in one non-infectious case (a false-positive case). In addition, there were two PCR false-negative cases that were clinically suspected of having fungal endophthalmitis.
conclusionsThis novel quantitative broad-range PCR of fungal 28 S rDNA is a useful tool for diagnosing endophthalmitis related to fungal infections.
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Registered trials
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