ArticleExtremophiles : life under extreme conditions2012
Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria.
Article in Extremophiles : life under extreme conditions, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 23 citations in OpenAlex.
- From fibres to adhesives: evolution of spider capture threads from web anchors by radical changes in silk gland function.Journal of the Royal Society, Interface · 2024Article
- Halophiles: biology, adaptation, and their role in decontamination of hypersaline environments.World journal of microbiology & biotechnology · 2016Review
- Detection of pigments of halophilic endoliths from gypsum: Raman portable instrument and European Space Agency's prototype analysis.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2014Article
- Raman spectroscopy in halophile research.Frontiers in microbiology · 2013Review
- Glycine betaine is the main organic osmotic solute in a stratified microbial community in a hypersaline evaporitic gypsum crust.Extremophiles : life under extreme conditions · 2013Article
- Microorganism response to stressed terrestrial environments: a Raman spectroscopic perspective of extremophilic life strategies.Life (Basel, Switzerland) · 2013Review
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We explored the use of Raman spectroscopy to detect organic osmotic solutes as biomarkers in the moderately halophilic heterotrophic bacterium Halomonas elongata grown in complex medium (accumulation of glycine betaine) and in defined medium with glucose as carbon source (biosynthesis of ectoine), and in the anoxygenic phototrophic Ectothiorhodospira marismortui known to synthesize glycine betaine in combination with minor amounts of trehalose and N-α-carbamoyl glutamineamide. We tested different methods of preparation of the material: lyophilization, two-phase extraction of water-soluble molecules, and perchlorate extraction. Raman signals of glycine betaine and ectoine were detected; perchlorate extraction followed by desalting the extract on an ion retardation column gave the best results. Lyophilized cells of E. marismortui showed strong signals of carotenoid pigments, and glycine betaine could be detected only after perchlorate extraction and desalting. The data presented show that Raman spectroscopy is a suitable tool to assess the mode of osmotic adaptation used by halophilic microorganisms.
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