ArticleExtremophiles : life under extreme conditions2012
Overexpression in a non-native halophilic host and biotechnological potential of NAD+-dependent glutamate dehydrogenase from Halobacterium salinarum strain NRC-36014.
Article in Extremophiles : life under extreme conditions, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 2 of them syntheses that pooled it.
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Who cites it
13 citing papers in PubMed, 2 syntheses or guidelines pooled it, 26 citations in OpenAlex.
- Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.Microbial cell factories · 2021Pooled it
- A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils.World journal of microbiology & biotechnology · 2013Pooled it
- Biochemical properties of glycerol kinase from the hypersaline-adapted archaeonApplied and environmental microbiology · 2025Article
- Potassium binding by carbonyl clusters, halophilic adaptation and catalysis of Haloferax mediterranei D-2-hydroxyacid dehydrogenase.Communications biology · 2025Article
- Exploring Oxidoreductases from Extremophiles for Biosynthesis in a Non-Aqueous System.International journal of molecular sciences · 2023Review
- Directed evolution engineering to improve activity of glucose dehydrogenase by increasing pocket hydrophobicity.Frontiers in microbiology · 2022Article
- Article
- Extremophilic Oxidoreductases for the Industry: Five Successful Examples With Promising Projections.Frontiers in bioengineering and biotechnology · 2021Review
- Reversible Activation of Halophilic β-lactamase from Methanol-Induced Inactive Form: Contrast to Irreversible Inactivation of Non-Halophilic Counterpart.The protein journal · 2017Article
- Biochemical characterization of two glutamate dehydrogenases with different cofactor specificities from a hyperthermophilic archaeon Pyrobaculum calidifontis.Extremophiles : life under extreme conditions · 2013Article
- Prospects for robust biocatalysis: engineering of novel specificity in a halophilic amino acid dehydrogenase.Extremophiles : life under extreme conditions · 2013Article
- Effect of organic solvents on the activity and stability of halophilic alcohol dehydrogenase (ADH2) from Haloferax volcanii.Extremophiles : life under extreme conditions · 2013Article
- Development of a colorimetric α-ketoglutarate detection assay for prolyl hydroxylase domain (PHD) proteins.The Journal of biological chemistryArticle
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Enzymes produced by halophilic archaea are generally heat resistant and organic solvent tolerant, and accordingly important for biocatalytic applications in 'green chemistry', frequently requiring a low-water environment. NAD(+)-dependent glutamate dehydrogenase from an extremely halophilic archaeon Halobacterium salinarum strain NRC-36014 was selected to explore the biotechnological potential of this enzyme and genetically engineered derivatives. Over-expression in a halophilic host Haloferax volcanii provided a soluble, active recombinant enzyme, not achievable in mesophilic Escherichia coli, and an efficient purification procedure was developed. pH and salt dependence, thermostability, organic solvent stability and kinetic parameters were explored. The enzyme is active up to 90 °C and fully stable up to 70 °C. It shows good tolerance of various miscible organic solvents. High concentrations of salt may be substituted with 30 % DMSO or betaine with good stability and activity. The robustness of this enzyme under a wide range of conditions offers a promising scaffold for protein engineering.
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