ArticleExtremophiles : life under extreme conditions2012
Validation of housekeeping genes for gene expression studies in an ice alga Chlamydomonas during freezing acclimation.
Article in Extremophiles : life under extreme conditions, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Antarctic MicroalgaMarine drugs · 2026Article
- Identification of housekeeping gene for future studies exploring effects of cryopreservation on gene expression in shrimp.Scientific reports · 2025Article
- Effect of Cryopreservation on Proteins from the Ubiquitous Marine DinoflagellatePlants (Basel, Switzerland) · 2021Article
- Article
- Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui.PloS one · 2021Article
- Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically TransformedLife (Basel, Switzerland) · 2020Article
- Molecular Cloning and Expression of a Cryptochrome Gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L.Molecular biotechnology · 2020Article
- Genome Improvement and Core Gene Set Refinement ofMicroorganisms · 2020Article
- Glycerol Is an Osmoprotectant in Two AntarcticFrontiers in plant science · 2020Article
- Simplified Transformation ofGenes · 2019Article
- Validation of reference genes for qRT-PCR data normalisation in lentil (Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML.Applied and environmental microbiology · 2018Article
- Molecular cloning and expression analysis of major intrinsic protein gene in Chlamydomonas sp. ICE-L from Antarctica.Extremophiles : life under extreme conditions · 2017Article
- Article
- Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp. JSC4.Scientific reports · 2017Article
- Validation of reference genes for cryopreservation studies with the gorgonian coral endosymbiont Symbiodinium.Scientific reports · 2017Article
- Article
- Acclimation of Antarctic Chlamydomonas to the sea-ice environment: a transcriptomic analysis.Extremophiles : life under extreme conditions · 2016Article
- Article
- Transcriptome-wide analysis of DEAD-box RNA helicase gene family in an Antarctic psychrophilic alga Chlamydomonas sp. ICE-L.Extremophiles : life under extreme conditions · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antarctic ice alga Chlamydomonas sp. ICE-L can endure extreme low temperature and high salinity stress under freezing conditions. To elucidate the molecular acclimation mechanisms using gene expression analysis, the expression stabilities of ten housekeeping genes of Chlamydomonas sp. ICE-L during freezing stress were analyzed. Some discrepancies were detected in the ranking of the candidate reference genes between geNorm and NormFinder programs, but there was substantial agreement between the groups of genes with the most and the least stable expression. RPL19 was ranked as the best candidate reference genes. Pairwise variation (V) analysis indicated the combination of two reference genes was sufficient for qRT-PCR data normalization under the experimental conditions. Considering the co-regulation between RPL19 and RPL32 (the most stable gene pairs given by geNorm program), we propose that the mean data rendered by RPL19 and GAPDH (the most stable gene pairs given by NormFinder program) be used to normalize gene expression values in Chlamydomonas sp. ICE-L more accurately. The example of FAD3 gene expression calculation demonstrated the importance of selecting an appropriate category and number of reference genes to achieve an accurate and reliable normalization of gene expression during freeze acclimation in Chlamydomonas sp. ICE-L.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.