ArticleApplied microbiology and biotechnology2012
Mining of unexplored habitats for novel chitinases--chiA as a helper gene proxy in metagenomics.
Article in Applied microbiology and biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- An artificial selection procedure enriches for known and suspected chitin degraders from the prokaryotic rare biosphere of multiple marine biotopes.BMC microbiology · 2025Article
- Exploring protein natural diversity in environmental microbiomes with DeepMetagenome.Cell reports methods · 2024Article
- Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Thermophilic Chitinases: Structural, Functional and Engineering Attributes for Industrial Applications.Applied biochemistry and biotechnology · 2021Review
- Harnessing the sponge microbiome for industrial biocatalysts.Applied microbiology and biotechnology · 2020Review
- Dynamics of biochemical properties associated with soil nitrogen mineralization following nitrification inhibitor and fungicide applications.Environmental science and pollution research international · 2017Article
- Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome.Microbial cell factories · 2017Article
- A broad pH range and processive chitinase from a metagenome library.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Article
- Microbial community structure of two freshwater sponges using Illumina MiSeq sequencing revealed high microbial diversity.AMB Express · 2016Article
- Different inocula produce distinctive microbial consortia with similar lignocellulose degradation capacity.Applied microbiology and biotechnology · 2016Article
- A novel salt-tolerant chitobiosidase discovered by genetic screening of a metagenomic library derived from chitin-amended agricultural soil.Applied microbiology and biotechnology · 2015Article
- Analysis of plant microbe interactions in the era of next generation sequencing technologies.Frontiers in plant science · 2014Review
- Chitin amendment increases soil suppressiveness toward plant pathogens and modulates the actinobacterial and oxalobacteraceal communities in an experimental agricultural field.Applied and environmental microbiology · 2013Article
- Bacterial chitin degradation-mechanisms and ecophysiological strategies.Frontiers in microbiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The main objective of this study was to assess the abundance and diversity of chitin-degrading microbial communities in ten terrestrial and aquatic habitats in order to provide guidance to the subsequent exploration of such environments for novel chitinolytic enzymes. A combined protocol which encompassed (1) classical overall enzymatic assays, (2) chiA gene abundance measurement by qPCR, (3) chiA gene pyrosequencing, and (4) chiA gene-based PCR-DGGE was used. The chiA gene pyrosequencing is unprecedented, as it is the first massive parallel sequencing of this gene. The data obtained showed the existence across habitats of core bacterial communities responsible for chitin assimilation irrespective of ecosystem origin. Conversely, there were habitat-specific differences. In addition, a suite of sequences were obtained that are as yet unregistered in the chitinase database. In terms of chiA gene abundance and diversity, typical low-abundance/diversity versus high-abundance/diversity habitats was distinguished. From the combined data, we selected chitin-amended agricultural soil, the rhizosphere of the Arctic plant Oxyria digyna and the freshwater sponge Ephydatia fluviatilis as the most promising habitats for subsequent bioexploration. Thus, the screening strategy used is proposed as a guide for further metagenomics-based exploration of the selected habitats.
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