ArticleJournal of clinical immunology2012
Calcitriol decreases expression of importin α3 and attenuates RelA translocation in human bronchial smooth muscle cells.
Article in Journal of clinical immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Impact of vitamin D supplementation on inflammatory markers in African Americans: results of a four-arm, randomized, placebo-controlled trial.Cancer prevention research (Philadelphia, Pa.) · 2014Trial
- An Integrated Machine Learning Framework for Developing and Validating a Diagnostic Model of Hub Genes Related to Lipid Metabolism in Chronic Rhinosinusitis.Journal of inflammation research · 2025Article
- Nutrition, Gut Microbiota, and Epigenetics in the Modulation of Immune Response and Metabolic Health.Cardiology and cardiovascular medicine · 2025Article
- Glucocorticoid Insensitivity in Severe Asthma: Underlying Molecular Mechanisms, Challenges, and Emerging Therapies.Archives of internal medicine research · 2025Article
- Karyopherins in the Remodeling of Extracellular Matrix: Implications in Tendon Injury.Journal of orthopaedics and sports medicine · 2023Article
- Article
- LncRNA ST7-AS1, by regulating miR-181b-5p/KPNA4 axis, promotes the malignancy of lung adenocarcinoma.Cancer cell international · 2020Article
- Asthmatic Patients with Vitamin D Deficiency have Decreased Exacerbations after Vitamin Replacement.Nutrients · 2017Observational
- miR-708-5p: a microRNA with emerging roles in cancer.Oncotarget · 2017Review
- Inhibition of KPNA4 attenuates prostate cancer metastasis.Oncogene · 2017Article
- Reduced miR‑26a and miR‑26b expression contributes to the pathogenesis of osteoarthritis via the promotion of p65 translocation.Molecular medicine reports · 2017Article
- Effect of active vitamin D3 on VEGF-induced ADAM33 expression and proliferation in human airway smooth muscle cells: implications for asthma treatment.Respiratory research · 2017Article
- Reanalysis of microRNA expression profiles identifies novel biomarkers for hepatocellular carcinoma prognosis.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Upregulation of a disintegrin and metalloproteinase-33 by VEGF in human airway smooth muscle cells: Implications for asthma.Cell cycle (Georgetown, Tex.) · 2016Article
- Vitamin D controls resistance artery function through regulation of perivascular adipose tissue hypoxia and inflammation.Journal of molecular and cellular cardiology · 2016Article
- Vitamin D Deficiency Accelerates Coronary Artery Disease Progression in Swine.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- The impact of vitamin D on asthmatic human airway smooth muscle.Expert review of respiratory medicine · 2016Article
- Vitamin D Supplementation Reduces Induction of Epithelial-Mesenchymal Transition in Allergen Sensitized and Challenged Mice.PloS one · 2016Article
- Vitamin D attenuates cytokine-induced remodeling in human fetal airway smooth muscle cells.Journal of cellular physiology · 2015Article
- Vitamin D Modulates Expression of the Airway Smooth Muscle Transcriptome in Fatal Asthma.PloS one · 2015Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
purposeA potent immunomodulatory role of Vitamin D in both innate and adaptive immunity has recently been appreciated. In allergic asthma, activation of NF-кB induces transcription of various cytokines and chemokines involved in allergic airway inflammation. The nuclear import of activated NF-кB p50/RelA subunit is dependent on importin α3 (KPNA4) and importin α4 (KPNA3). In this study, we examined the role of importin α3 in immunomodulatory effect of calcitriol in human bronchial smooth muscle cells (HBSMCs).
methodsCultured HBSMCs were stimulated with calcitriol in the presence and absence of cytokines, TNF-α, IL-1β, and IL-10. The mRNA transcripts of importin α3 and α4 were analyzed using qPCR while protein expression of importin α3, α4 and nuclear RelA was analyzed by immunoblotting.
resultsCalcitriol significantly decreased mRNA and protein expression of importin α3 as well as nuclear protein expression of NF-кB p65 (RelA). The decreased activation of RelA by calcitriol was confirmed by decreased release of RelA-inducible molecules, including IL-5, IL-6 and IL-8, by HBSMCs upon calcitriol treatment. Calcitriol attenuated the effect of TNF-α and IL-1β to upregulate mRNA and protein expression of importin α3. IL-10 significantly decreased the TNF-α induced expression of importin α3 and this effect was further potentiated by calcitriol.
conclusionsThese data suggest that under inflammatory conditions, calcitriol decreases the expression of importin α3 resulting in decreased nuclear import of activated RelA. This could be a novel mechanism by which calcitriol could exert its immunomodulatory effects to reduce allergic airway inflammation and thus may alleviate the symptoms in allergic asthma.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.