ArticlePlanta2012
Genome-wide identification of microRNAs in larch and stage-specific modulation of 11 conserved microRNAs and their targets during somatic embryogenesis.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 141 citations in OpenAlex.
- Conserved miR156 Mediates Phase-Specific Coordination Between Cotyledon Morphogenesis and Embryo Dormancy During Somatic Embryogenesis inInternational journal of molecular sciences · 2025Article
- Identification of Target Gene and Interacting Protein of TwoPlants (Basel, Switzerland) · 2024Article
- miR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways.International journal of molecular sciences · 2024Article
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- Characterization of microRNAs and Target Genes inPlants (Basel, Switzerland) · 2023Article
- Construction of ceRNA Networks at Different Stages of Somatic Embryogenesis in Garlic.International journal of molecular sciences · 2023Article
- Identification ofForestry research · 2023Article
- Genetic and epigenetic modes of the regulation of somatic embryogenesis: a review.Biologia futura · 2022Review
- miR171 modulates induction of somatic embryogenesis in citrus callus.Plant cell reports · 2022Article
- Global DNA Methylation and mRNA-miRNA Variations Activated by Heat Shock Boost Early Microspore Embryogenesis in Cabbage (International journal of molecular sciences · 2022Article
- Function and Regulation of microRNA171 in Plant Stem Cell Homeostasis and Developmental Programing.International journal of molecular sciences · 2022Review
- Identification and profiling of conserved microRNAs in different developmental stages of crown imperial (Fritillaria imperialis L.) using high-throughput sequencing.Molecular biology reports · 2022Article
- Integrated transcriptomic and metabolic analyses provide insights into the maintenance of embryogenic potential and the biosynthesis of phenolic acids and flavonoids involving transcription factors inFrontiers in plant science · 2022Article
- Regulatory non-coding RNAs: Emerging roles during plant cell reprogramming andFrontiers in plant science · 2022Review
- Mini review: Application of the somatic embryogenesis technique in conifer species.Forestry research · 2022Review
- miR160 InteractsFrontiers in plant science · 2022Article
- Small Non-Coding RNAs at the Crossroads of Regulatory Pathways Controlling Somatic Embryogenesis in Seed Plants.Plants (Basel, Switzerland) · 2021Review
- Molecular characterization of miRNA genes and their expression in Dimocarpus longan Lour.Planta · 2021Article
- Embryogenic callus induction from immature zygotic embryos and genetic transformation of Larix kaempferi 3x Larix gmelinii 9.PloS one · 2021Article
- miRNAs as key regulators via targeting the phytohormone signaling pathways during somatic embryogenesis of plants.3 Biotech · 2020Review
7 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are emerging as essential regulators of biological processes. Somatic embryogenesis is one of the most important techniques for gymnosperm-breeding programs, but there is little understanding of its underlying mechanism. To investigate the roles of miRNAs during somatic embryogenesis in larch, we constructed a small RNA library from somatic embryos. High-throughput sequencing of the library identified 83 conserved miRNAs from 35 families, 16 novel miRNAs, and 14 plausible miRNA candidates, with a high proportion specific to larch or gymnosperms. qRT-PCR analysis demonstrated that both the conserved and novel or candidate miRNAs were expressed in larch. Several miRNA precursor sequences were obtained via RACE. We predicted 110 target genes using bioinformatics, and validated 9 of them by 5' RACE. 11 conserved miRNA families including 17 miRNAs with critical functions in plant development and six target mRNAs were detected by qRT-PCR in the larch SE. Stage-specific expression of miRNAs and their targets indicate their possible modulation on SE of larch: miR171a/b might exert function on PEMs, while miR171c acts in the induction process of larch SE; miR397 and miR398 mainly involved in modulation of PEM propagation and transition to single embryo; miR162 and miR168 exert their regulatory function during total SE process, especially during stages 5-8; miR156, miR159, miR160, miR166, miR167, and miR390 might play regulatory roles during cotyledonary embryo development. These findings indicate that larch and possibly other gymnosperms have complex mechanisms of gene regulation involving specific and common miRNAs operating post-transcriptionally during embryogenesis.
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