ArticleJournal of visualized experiments : JoVE2012
Phenotypic and functional characterization of endothelial colony forming cells derived from human umbilical cord blood.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- Endothelial progenitor cells for fabrication of engineered vascular units and angiogenesis induction.Cell proliferation · 2024Review
- Cord Blood-Derived Endothelial Progenitor Cells Promote In Vivo Regeneration of Human Hematopoietic Bone Marrow.International journal of radiation oncology, biology, physics · 2023Article
- Acetate controls endothelial-to-mesenchymal transition.Cell metabolism · 2023Article
- Pathological Roles for Endothelial Colony-Forming Cells in Neonatal and Adult Lung Disease.American journal of respiratory cell and molecular biology · 2023Review
- Heterotypic Multicellular Spheroids as Experimental and Preclinical Models of Sprouting Angiogenesis.Biology · 2021Review
- Proteomic Signature of Dysfunctional Circulating Endothelial Colony-Forming Cells of Young Adults.Journal of the American Heart Association · 2021Article
- Comprehensive phenotyping of endothelial cells using flow cytometry 2: Human.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Advances of Endothelial Progenitor Cells in the Development of Depression.Frontiers in cellular neuroscience · 2021Review
- Therapeutic Potential of Endothelial Colony-Forming Cells in Ischemic Disease: Strategies to Improve their Regenerative Efficacy.International journal of molecular sciences · 2020Review
- Review
- O-GlcNAc Transferase Regulates Angiogenesis in Idiopathic Pulmonary Arterial Hypertension.International journal of molecular sciences · 2019Article
- Dickkopf-1 Treatment Stimulates Hematopoietic Regenerative Function in Infused Endothelial Progenitor Cells.Radiation research · 2019Article
- Significance of Cellular Cross-Talk in Stromal Vascular Fraction of Adipose Tissue in Neovascularization.Arteriosclerosis, thrombosis, and vascular biology · 2019Review
- Panobinostat, a histone deacetylase inhibitor, rescues the angiogenic potential of endothelial colony-forming cells in moyamoya disease.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2019Article
- The Use of Live Cell Imaging and Automated Image Analysis to Assist With Determining Optimal Parameters for Angiogenic AssayFrontiers in cell and developmental biology · 2019Article
- Endothelial Colony-Forming Cells in Young Adults Born Preterm: A Novel Link Between Neonatal Complications and Adult Risks for Cardiovascular Disease.Journal of the American Heart Association · 2018Observational
- Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro.Journal of visualized experiments : JoVE · 2018Article
- Preclinical Evaluation and Optimization of a Cell Therapy Using Human Cord Blood-Derived Endothelial Colony-Forming Cells for Ischemic Retinopathies.Stem cells translational medicine · 2018Article
- Epigenetic Activation of Pro-angiogenic Signaling Pathways in Human Endothelial Progenitors Increases Vasculogenesis.Stem cell reports · 2017Article
- Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Longstanding views of new blood vessel formation via angiogenesis, vasculogenesis, and arteriogenesis have been recently reviewed. The presence of circulating endothelial progenitor cells (EPCs) were first identified in adult human peripheral blood by Asahara et al. in 1997 bringing an infusion of new hypotheses and strategies for vascular regeneration and repair. EPCs are rare but normal components of circulating blood that home to sites of blood vessel formation or vascular remodeling, and facilitate either postnatal vasculogenesis, angiogenesis, or arteriogenesis largely via paracrine stimulation of existing vessel wall derived cells. No specific marker to identify an EPC has been identified, and at present the state of the field is to understand that numerous cell types including proangiogenic hematopoietic stem and progenitor cells, circulating angiogenic cells, Tie2+ monocytes, myeloid progenitor cells, tumor associated macrophages, and M2 activated macrophages participate in stimulating the angiogenic process in a variety of preclinical animal model systems and in human subjects in numerous disease states. Endothelial colony forming cells (ECFCs) are rare circulating viable endothelial cells characterized by robust clonal proliferative potential, secondary and tertiary colony forming ability upon replating, and ability to form intrinsic in vivo vessels upon transplantation into immunodeficient mice. While ECFCs have been successfully isolated from the peripheral blood of healthy adult subjects, umbilical cord blood (CB) of healthy newborn infants, and vessel wall of numerous human arterial and venous vessels. CB possesses the highest frequency of ECFCs that display the most robust clonal proliferative potential and form durable and functional blood vessels in vivo. While the derivation of ECFC from adult peripheral blood has been presented, here we describe the methodologies for the derivation, cloning, expansion, and in vitro as well as in vivo characterization of ECFCs from the human umbilical CB.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.