ArticleActa crystallographica. Section D, Biological crystallography2012
In situ macromolecular crystallography using microbeams.
Article in Acta crystallographica. Section D, Biological crystallography, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
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Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Structure Elucidation of Coxsackievirus A16 in Complex with GPP3 Informs a Systematic Review of Highly Potent Capsid Binders to Enteroviruses.PLoS pathogens · 2015Pooled it
- A user-friendly goniometer-compatible fixed-target platform for macromolecular crystallography at synchrotrons.Journal of applied crystallography · 2026Article
- In situ serial crystallography facilitates 96-well plate structural analysis at low symmetry.IUCrJ · 2024Article
- Efficient in situ screening of and data collection from microcrystals in crystallization plates.Acta crystallographica. Section D, Structural biology · 2024Article
- Integrative Approaches to Study Virus Structures.Sub-cellular biochemistry · 2024Review
- X-Ray Crystallography of Viruses.Sub-cellular biochemistry · 2024Review
- xia2.multiplex: a multi-crystal data-analysis pipeline.Acta crystallographica. Section D, Structural biology · 2022Article
- In situ crystal data-collection and ligand-screening system at SPring-8.Acta crystallographica. Section F, Structural biology communications · 2022Article
- A capillary-based microfluidic device enables primary high-throughput room-temperature crystallographic screening.Journal of applied crystallography · 2021Article
- Implementation of level-2 biosafety for a macromolecular crystallography beamline at SSRF.American journal of nuclear medicine and molecular imaging · 2021Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- An automated platform forIUCrJ · 2020Article
- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- Scaling diffraction data in the DIALS software package: algorithms and new approaches for multi-crystal scaling.Acta crystallographica. Section D, Structural biology · 2020Article
- Glutathione facilitates enterovirus assembly by binding at a druggable pocket.Communications biology · 2020Article
- Visualization of protein crystals by high-energy phase-contrast X-ray imaging.Acta crystallographica. Section D, Structural biology · 2019Article
- Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation.Scientific reports · 2019Article
- Dose-resolved serial synchrotron and XFEL structures of radiation-sensitive metalloproteins.IUCrJ · 2019Article
- Hepatitis A Virus Capsid Structure.Cold Spring Harbor perspectives in medicine · 2019Review
- Making routine native SAD a reality: lessons from beamline X06DA at the Swiss Light Source.Acta crystallographica. Section D, Structural biology · 2019Article
15 more citing papers are in PubMed but not listed here.
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18 authors.
Funding
Abstract
Despite significant progress in high-throughput methods in macromolecular crystallography, the production of diffraction-quality crystals remains a major bottleneck. By recording diffraction in situ from crystals in their crystallization plates at room temperature, a number of problems associated with crystal handling and cryoprotection can be side-stepped. Using a dedicated goniometer installed on the microfocus macromolecular crystallography beamline I24 at Diamond Light Source, crystals have been studied in situ with an intense and flexible microfocus beam, allowing weakly diffracting samples to be assessed without a manual crystal-handling step but with good signal to noise, despite the background scatter from the plate. A number of case studies are reported: the structure solution of bovine enterovirus 2, crystallization screening of membrane proteins and complexes, and structure solution from crystallization hits produced via a high-throughput pipeline. These demonstrate the potential for in situ data collection and structure solution with microbeams.
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