ArticleJournal of visualized experiments : JoVE2012
High-resolution live imaging of cell behavior in the developing neuroepithelium.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Cellular signalling protrusions enable dynamic distant contacts in spinal cord neurogenesis.Biology open · 2025Article
- A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis.Journal of cell science · 2022Article
- Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.Science advances · 2020Article
- Embryonic Chicken (Comparative medicine · 2019Review
- Organotypic slice culture based on in ovo electroporation for chicken embryonic central nervous system.Journal of cellular and molecular medicine · 2019Article
- Article
- Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin.Nature communications · 2016Article
- Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.Science (New York, N.Y.) · 2014Article
- A method to investigate radial glia cell behavior using two-photon time-lapse microscopy in an ex vivo model of spinal cord development.Frontiers in neuroanatomy · 2014Article
- Chicken embryo spinal cord slice culture protocol.Journal of visualized experiments : JoVE · 2013Article
- Mitotic spindle orientation can direct cell fate and bias Notch activity in chick neural tube.EMBO reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The embryonic spinal cord consists of cycling neural progenitor cells that give rise to a large percentage of the neuronal and glial cells of the central nervous system (CNS). Although much is known about the molecular mechanisms that pattern the spinal cord and elicit neuronal differentiation, we lack a deep understanding of these early events at the level of cell behavior. It is thus critical to study the behavior of neural progenitors in real time as they undergo neurogenesis. In the past, real-time imaging of early embryonic tissue has been limited by cell/tissue viability in culture as well as the phototoxic effects of fluorescent imaging. Here we present a novel assay for imaging such tissue for long periods of time, utilizing a novel ex vivo slice culture protocol and wide-field fluorescence microscopy (Fig. 1). This approach achieves long-term time-lapse monitoring of chick embryonic spinal cord progenitor cells with high spatial and temporal resolution. This assay may be modified to image a range of embryonic tissues. In addition to the observation of cellular and sub-cellular behaviors, the development of novel and highly sensitive reporters for gene activity (for example, Notch signaling) makes this assay a powerful tool with which to understand how signaling regulates cell behavior during embryonic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.