ArticleMolecular plant pathology2012
Redox-sensitive GFP2: use of the genetically encoded biosensor of the redox status in the filamentous fungus Botrytis cinerea.
Article in Molecular plant pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Cytokinin Regulates Energy Utilization in Botrytis cinerea.Microbiology spectrum · 2022Article
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Identification of genes involved in fungal responses to strigolactones using mutants from fungal pathogens.Current genetics · 2017Article
- Secretory proteins are delivered to the septin-organized penetration interface during root infection by Verticillium dahliae.PLoS pathogens · 2017Article
- Optimization of the HyPer sensor for robust real-time detection of hydrogen peroxide in the rice blast fungus.Molecular plant pathology · 2017Article
- The Protein Disulfide Isomerase ofFrontiers in microbiology · 2017Article
- Proteomic Analysis of Rhizoctonia solani Identifies Infection-specific, Redox Associated Proteins and Insight into Adaptation to Different Plant Hosts.Molecular & cellular proteomics : MCP · 2016Article
- Update on Nox function, site of action and regulation inFungal biology and biotechnology · 2016Article
- Real-time imaging of hydrogen peroxide dynamics in vegetative and pathogenic hyphae of Fusarium graminearum.Scientific reports · 2015Article
- Systematic characterization of the peroxidase gene family provides new insights into fungal pathogenicity in Magnaporthe oryzae.Scientific reports · 2015Article
- Unraveling the Function of the Response Regulator BcSkn7 in the Stress Signaling Network of Botrytis cinerea.Eukaryotic cell · 2015Article
- The transcription factor BcLTF1 regulates virulence and light responses in the necrotrophic plant pathogen Botrytis cinerea.PLoS genetics · 2014Article
- Role of the transcription factor ChAP1 in cytoplasmic redox homeostasis: imaging with a genetically encoded sensor in the maize pathogen Cochliobolus heterostrophus.Molecular plant pathology · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of reactive oxygen species (ROS) is part of the defence reaction of plants against invading pathogens. The effect of ROS on filamentous fungi is still unclear. In this study, ratiometric redox-sensitive green fluorescent protein (roGFP) was introduced as a tool for in vivo measurement of the cellular redox status in filamentous fungi. A fungal expression system for roGFP2 was constructed. Expressed in Botrytis cinerea, roGFP2 reversibly responded to redox changes induced by incubation with H(2)O(2) or dithiothreitol, which was determined by confocal laser scanning microscopy imaging and fluorometry. As the sensor detects the redox potential of the cellular glutathione pool, it was used to analyse the kinetics of GSH (glutathione, reduced form) recovery after H(2)O(2) treatment. The transcription factor Bap1 is the main transcriptional regulator of H(2)O(2) -scavenging proteins in B. cinerea. When compared with the wild-type, GSH recovery in the Δbap1 deletion mutant was affected after repeated H(2)O(2) treatment. ROS and intracellular redox changes can be used by fungi for signalling purposes. In planta experiments, performed in this study, indicated that redox processes seem to be important for the differentiation of penetration structures. During the penetration of onion epidermal cells, the status of the cellular glutathione pool differed between appressoria-like structures and infecting hyphae, being reduced in the presence of infecting hyphae and more oxidized around appressoria-like structures.
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