ArticleJournal of bacteriology2012
The response regulator RcsB activates expression of Mat fimbriae in meningitic Escherichia coli.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Contrasting regulation of the ecp common pilus operon in Citrobacter rodentium: essential roles of a distal element and IHF even in the absence of H-NS.Archives of microbiology · 2026Article
- RcsB and H-NS Both Contribute to the Repression the Expression of theMicroorganisms · 2025Article
- Whole-genome analysis of fiveMicrobiology spectrum · 2025Article
- Mechanism ofInternational journal of molecular sciences · 2023Article
- Skin dysbiosis and Cutibacterium acnes biofilm in inflammatory acne lesions of adolescents.Scientific reports · 2022Article
- A Novel LysR Family Factor STM0859 is Associated with The Responses ofPolish journal of microbiology · 2021Article
- Regulation of gene expression by non-phosphorylated response regulators.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- The impact of Hfq-mediated sRNA-mRNA interactome on the virulence of enteropathogenicScience advances · 2021Article
- Structure-based analyses of Salmonella RcsB variants unravel new features of the Rcs regulon.Nucleic acids research · 2021Article
- Pathogenicity Factors of Genomic Islands in Intestinal and ExtraintestinalFrontiers in microbiology · 2020Review
- RcsAB and Fur Coregulate the Iron-Acquisition System viaFrontiers in cellular and infection microbiology · 2020Article
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- The Rcs regulon in Proteus mirabilis: implications for motility, biofilm formation, and virulence.Current genetics · 2016Article
- Interaction of the RcsB Response Regulator with Auxiliary Transcription Regulators in Escherichia coli.The Journal of biological chemistry · 2016Article
- Novel vaccine antigen combinations elicit protective immune responses against Escherichia coli sepsis.Vaccine · 2016Article
- Mutations That Stimulate flhDC Expression in Escherichia coli K-12.Journal of bacteriology · 2015Article
- Conserved aspartate and lysine residues of RcsB are required for amylovoran biosynthesis, virulence, and DNA binding in Erwinia amylovora.Molecular genetics and genomics : MGG · 2015Article
- The Intimin-Like Protein FdeC Is Regulated by H-NS and Temperature in Enterohemorrhagic Escherichia coli.Applied and environmental microbiology · 2014Article
- Escherichia coli isolate for studying colonization of the mouse intestine and its application to two-component signaling knockouts.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The common colonization factor of Escherichia coli, the Mat (also termed ECP) fimbria, functions to advance biofilm formation on inert surfaces as well as bacterial adherence to epithelial cells and subsequent colonization. We used global mini-Tn5 transposon mutagenesis to identify novel regulators of biofilm formation by the meningitic E. coli isolate IHE 3034. Of the 4,418 transformants, we found 17 that were impaired in biofilm formation. Most of these mutants were affected in lipopolysaccharide synthesis and were reduced in growth but not in Mat fimbria expression. In contrast, two mutants grew well but did not express Mat fimbria. The insertions in these two mutants were located at different sites of the rcsB gene, which encodes a DNA-binding response regulator of the Rcs response regulon. The mutations abrogated temperature-dependent biofilm formation by IHE 3034, and the phenotype correlated with loss of mat expression. The defect in biofilm formation in the rcsB mutant was reversed upon complementation with rcsB as well as by overexpression of structural mat genes but not by overexpression of the fimbria-specific activator gene matA. Monitoring of the mat operon promoter activity with chromosomal reporter fusions showed that the RcsB protein and an RcsAB box in the mat regulatory region, but not RcsC, RcsD, AckA, and Pta, are essential for initiation of mat transcription. Gel retardation assays showed that RcsB specifically binds to the mat promoter DNA, which enables its function in promoting biofilm formation by E. coli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.