ArticleJournal of bacteriology2012
Effects of the SpoVT regulatory protein on the germination and germination protein levels of spores of Bacillus subtilis.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- L-Alanine promotes anti-infectious properties of Bacillus subtilis S-2 spores via the germination receptor gerAA.Probiotics and antimicrobial proteins · 2024Article
- Changes in the Spore Proteome ofMicroorganisms · 2022Article
- Effect of oxygen on the germination and culturability of Bacillus atrophaeus spores.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- The small acid-soluble proteins of Clostridioides difficile are important for UV resistance and serve as a check point for sporulation.PLoS pathogens · 2021Article
- ORF4 of the Temperate Archaeal Virus SNJ1 Governs the Lysis-Lysogeny Switch and Superinfection Immunity.Journal of virology · 2020Article
- Identification of L-Valine-initiated-germination-active genes in Bacillus subtilis using Tn-seq.PloS one · 2019Article
- SpoVT: From Fine-Tuning Regulator inFrontiers in microbiology · 2016Article
- The effects of heat activation on Bacillus spore germination, with nutrients or under high pressure, with or without various germination proteins.Applied and environmental microbiology · 2015Article
- Analysis of the dynamics of a Bacillus subtilis spore germination protein complex during spore germination and outgrowth.Journal of bacteriology · 2015Article
- Article
- Function of the SpoVAEa and SpoVAF proteins of Bacillus subtilis spores.Journal of bacteriology · 2014Article
- The effects of extremes of pH on the growth and transcriptomic profiles of three haloarchaea.F1000Research · 2014Article
- Live-cell imaging tool optimization to study gene expression levels and dynamics in single cells of Bacillus cereus.Applied and environmental microbiology · 2013Article
- Numbers of individual nutrient germinant receptors and other germination proteins in spores of Bacillus subtilis.Journal of bacteriology · 2013Article
- Identification of new proteins that modulate the germination of spores of bacillus species.Journal of bacteriology · 2013Article
- A novel RNA polymerase-binding protein controlling genes involved in spore germination in Bacillus subtilis.Molecular microbiology · 2013Article
- Investigating the functional hierarchy of Bacillus megaterium PV361 spore germinant receptors.Journal of bacteriology · 2013Article
- Genome-wide analysis of cell type-specific gene transcription during spore formation in Clostridium difficile.PLoS genetics · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis isolates lacking the SpoVT protein, which regulates gene expression in developing forespores, gave spores that released their dipicolinic acid (DPA) via germinant receptor (GR)-dependent germination more rapidly than wild-type spores. Non-GR-dependent germination via dodecylamine was more rapid with spoVT spores, but germination via Ca-DPA was slower. The effects of a spoVT mutation on spore germination were seen with spores made in rich and poor media, and levels of SpoVT-LacZ were elevated 2-fold in poor-medium spores; however, elevated SpoVT levels were not the only cause of the slower GR-dependent germination of poor-medium spores. The spoVT spores had ≥5-fold higher GerA GR levels, ∼2-fold elevated GerB GR levels, wild-type levels of a GerK GR subunit and the GerD protein required for normal GR-dependent germination, ∼2.5-fold lower levels of the SpoVAD protein involved in DPA release in spore germination, and 30% lower levels of DNA protective α/β-type small, acid-soluble spore proteins. With one exception, the effects on protein levels in spoVT spores are consistent with the effects of SpoVT on forespore transcription. The spoVT spores were also more sensitive to UV radiation and outgrew slowly. While spoVT spores' elevated GR levels were consistent with their more rapid GR-dependent germination, detailed analysis of the results suggested that there is another gene product crucial for GR-dependent spore germination that is upregulated in the absence of SpoVT. Overall, these results indicate that SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores.
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