ArticleJournal of bacteriology2012
Natural transformation of an engineered Helicobacter pylori strain deficient in type II restriction endonucleases.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- High-throughput conjugation reveals strain specific recombination patterns enabling precise trait mapping in Escherichia coli.PLoS genetics · 2025Article
- DNA modifications impact natural transformation of Acinetobacter baumannii.Nucleic acids research · 2023Article
- Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution.Science (New York, N.Y.) · 2022Article
- Natural Transformation ofFrontiers in microbiology · 2021Article
- The Clustered Regularly Interspaced Short Palindromic Repeat System and Argonaute: An Emerging Bacterial Immunity System for Defense Against Natural Transformation?Frontiers in microbiology · 2020Review
- Complete genome sequence of Helicobacter pylori B128 7.13 and a single-step method for the generation of unmarked mutations.Helicobacter · 2019Article
- Antibacterial Strategy againstACS medicinal chemistry letters · 2019Article
- N4-cytosine DNA methylation regulates transcription and pathogenesis in Helicobacter pylori.Nucleic acids research · 2018Article
- Metagenomic binning and association of plasmids with bacterial host genomes using DNA methylation.Nature biotechnology · 2018Article
- Genome-wide analysis of chromosomal import patterns after natural transformation of Helicobacter pylori.Nature communications · 2016Article
- Addiction of Hypertransformable Pneumococcal Isolates to Natural Transformation for In Vivo Fitness and Virulence.Infection and immunity · 2016Article
- Genetic Diversity as Consequence of a Microaerobic and Neutrophilic Lifestyle.PLoS pathogens · 2016Article
- Single molecule-level detection and long read-based phasing of epigenetic variations in bacterial methylomes.Nature communications · 2015Article
- A specific A/T polymorphism in Western tyrosine phosphorylation B-motifs regulates Helicobacter pylori CagA epithelial cell interactions.PLoS pathogens · 2015Article
- Phylogeographic evidence of cognate recognition site patterns and transformation efficiency differences in H. pylori: theory of strain dominance.BMC microbiology · 2013Article
- Helicobacter pylori DprA alleviates restriction barrier for incoming DNA.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Restriction-modification (RM) systems are important for bacteria to limit foreign DNA invasion. The naturally competent bacterium Helicobacter pylori has highly diverse strain-specific type II systems. To evaluate the roles of strain-specific restriction in H. pylori natural transformation, a markerless type II restriction endonuclease-deficient (REd) mutant was constructed. We deleted the genes encoding all four active type II restriction endonucleases in H. pylori strain 26695 using sacB-mediated counterselection. Transformation by donor DNA with exogenous cassettes methylated by Escherichia coli was substantially (1.7 and 2.0 log(10) for cat and aphA, respectively) increased in the REd strain. There also was significantly increased transformation of the REd strain by donor DNA from other H. pylori strains, to an extent corresponding to their shared type II R-M system strain specificity with 26695. Comparison of the REd and wild-type strains indicates that restriction did not affect the length of DNA fragment integration during natural transformation. There also were no differentials in cell growth or susceptibility to DNA damage. In total, the data indicate that the type II REd mutant has enhanced competence with no loss of growth or repair facility compared to the wild type, facilitating H. pylori mutant construction and other genetic engineering.
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