ArticleProteomics2012
Deep metaproteomic analysis of human salivary supernatant.
Article in Proteomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- Therapeutic Strategies and Genetic Implications for Periodontal Disease Management: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- A Viral Mutation Profiling and Discovery Strategy for Sensitive Multiplex Detection of Viruses and Variants in Saliva by Proteomics.bioRxiv : the preprint server for biology · 2026Article
- [Microbial metaproteomics--From sample processing to data acquisition and analysis].Se pu = Chinese journal of chromatography · 2024Review
- Marine-derived κ-carrageenan-coated zinc oxide nanoparticles for targeted drug delivery and apoptosis induction in oral cancer.Molecular biology reports · 2024Article
- The Evolving Microbiome of Dental Caries.Microorganisms · 2024Review
- A bibliometric analysis of the global impact of metaproteomics research.Frontiers in microbiology · 2023Article
- Current progress and critical challenges to overcome in the bioinformatics of mass spectrometry-based metaproteomics.Computational and structural biotechnology journal · 2023Review
- Candidate Phyla Radiation, an Underappreciated Division of the Human Microbiome, and Its Impact on Health and Disease.Clinical microbiology reviews · 2022Review
- Impact of different oral treatments on the composition of the supragingival plaque microbiome.Journal of oral microbiology · 2022Article
- Current State and Challenges of the Global Outcomes of Dental Caries Research in the Meta-Omics Era.Frontiers in cellular and infection microbiology · 2022Review
- Human body-fluid proteome: quantitative profiling and computational prediction.Briefings in bioinformatics · 2021Review
- Bottom-Up Community Proteome Analysis of Saliva Samples and Tongue Swabs by Data-Dependent Acquisition Nano LC-MS/MS Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Metaproteomics of the human gut microbiota: Challenges and contributions to other OMICS.Clinical mass spectrometry (Del Mar, Calif.) · 2019Review
- Progress and Challenges in Ocean Metaproteomics and Proposed Best Practices for Data Sharing.Journal of proteome research · 2019Review
- Metaproteomics analysis of microbial diversity of human saliva and tongue dorsum in young healthy individuals.Journal of oral microbiology · 2019Article
- High-throughput proteomic analysis of candidate biomarker changes in gingival crevicular fluid after treatment of chronic periodontitis.Journal of periodontal research · 2018Article
- Evaluating the potential of residual Pap test fluid as a resource for the metaproteomic analysis of the cervical-vaginal microbiome.Scientific reports · 2018Article
- Disseminating Metaproteomic Informatics Capabilities and Knowledge Using the Galaxy-P Framework.Proteomes · 2018Article
- Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva.Journal of oral microbiology · 2018Article
- Salivary biomarkers and proteomics: future diagnostic and clinical utilities.Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale · 2017Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The human salivary proteome is extremely complex, including proteins from salivary glands, serum, and oral microbes. Much has been learned about the host component, but little is known about the microbial component. Here we report a metaproteomic analysis of salivary supernatant pooled from six healthy subjects. For deep interrogation of the salivary proteome, we combined protein dynamic range compression (DRC), multidimensional peptide fractionation, and high-mass accuracy MS/MS with a novel two-step peptide identification method using a database of human proteins plus those translated from oral microbe genomes. Peptides were identified from 124 microbial species as well as uncultured phylotypes such as TM7. Streptococcus, Rothia, Actinomyces, Prevotella, Neisseria, Veilonella, Lactobacillus, Selenomonas, Pseudomonas, Staphylococcus, and Campylobacter were abundant among the 65 genera from 12 phyla represented. Taxonomic diversity in our study was broadly consistent with metagenomic studies of saliva. Proteins mapped to 20 KEGG pathways, with carbohydrate metabolism, amino acid metabolism, energy metabolism, translation, membrane transport, and signal transduction most represented. The communities sampled appear to be actively engaged in glycolysis and protein synthesis. This first deep metaproteomic catalog from human salivary supernatant provides a baseline for future studies of shifts in microbial diversity and protein activities potentially associated with oral disease.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.