ArticleGenome research2012
Precision genome engineering with programmable DNA-nicking enzymes.
Article in Genome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed.
- Multi-targeting zinc finger nuclease vector unsilences paternal UBE3A in a mouse model of Angelman syndrome.Gene therapy · 2026Article
- Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells.Nature communications · 2023Article
- Advances in S gene targeted genome-editing and its applicability to disease resistance breeding in selectedBioengineered · 2022Article
- Tools for Efficient Genome Editing; ZFN, TALEN, and CRISPR.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Different DNA repair pathways are involved in single-strand break-induced genomic changes in plants.The Plant cell · 2021Article
- Article
- Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species.Nucleic acids research · 2020Article
- Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife.Synthetic biology (Oxford, England) · 2020Review
- Specific targeting of point mutations in EGFR L858R-positive lung cancer by CRISPR/Cas9.Laboratory investigation; a journal of technical methods and pathology · 2018Article
- Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle.Scientific reports · 2017Article
- Control of gene editing by manipulation of DNA repair mechanisms.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- A Prospective Treatment Option for Lysosomal Storage Diseases: CRISPR/Cas9 Gene Editing Technology for Mutation Correction in Induced Pluripotent Stem Cells.Diseases (Basel, Switzerland) · 2017Review
- Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering.Computational and structural biotechnology journal · 2017Review
- Genome-Editing Technologies: Principles and Applications.Cold Spring Harbor perspectives in biology · 2016Review
- Genome editing comes of age.Nature protocols · 2016Review
- Enhanced genome editing in mammalian cells with a modified dual-fluorescent surrogate system.Cellular and molecular life sciences : CMLS · 2016Article
- DNA-free genome editing methods for targeted crop improvement.Plant cell reports · 2016Review
- Gene targeting, genome editing: from Dolly to editors.Transgenic research · 2016Review
- Origins of Programmable Nucleases for Genome Engineering.Journal of molecular biology · 2016Review
- Generation of TALE nickase-mediated gene-targeted cows expressing human serum albumin in mammary glands.Scientific reports · 2016Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc finger nucleases (ZFNs) are powerful tools of genome engineering but are limited by their inevitable reliance on error-prone nonhomologous end-joining (NHEJ) repair of DNA double-strand breaks (DSBs), which gives rise to randomly generated, unwanted small insertions or deletions (indels) at both on-target and off-target sites. Here, we present programmable DNA-nicking enzymes (nickases) that produce single-strand breaks (SSBs) or nicks, instead of DSBs, which are repaired by error-free homologous recombination (HR) rather than mutagenic NHEJ. Unlike their corresponding nucleases, zinc finger nickases allow site-specific genome modifications only at the on-target site, without the induction of unwanted indels. We propose that programmable nickases will be of broad utility in research, medicine, and biotechnology, enabling precision genome engineering in any cell or organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.