ArticleAnalytical chemistry2012
Tag-free microfluidic separation of cells against multiple markers.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Single-cell Technology in Stem Cell Research.Current stem cell research & therapy · 2025Review
- Microfluidics as efficient technology for the isolation and characterization of stem cells.EXCLI journal · 2021Review
- Microfluidic capture of endothelial colony-forming cells from human adult peripheral blood: phenotypic and functional validation in vivo.Tissue engineering. Part C, Methods · 2015Article
- Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.Reports on progress in physics. Physical Society (Great Britain) · 2015Review
- Perspective on microfluidic cell separation: a solved problem?Analytical chemistry · 2014Review
- Microfluidic Isolation of CD34-Positive Skin Cells Enables Regeneration of Hair and Sebaceous Glands In Vivo.Stem cells translational medicine · 2014Article
- Rare cell isolation and analysis in microfluidics.Lab on a chip · 2014Review
- Advances in microfluidic cell separation and manipulation.Current opinion in chemical engineering · 2013Article
- Microfluidic enrichment of mouse epidermal stem cells and validation of stem cell proliferation in vitro.Tissue engineering. Part C, Methods · 2013Article
- Recent advances in microfluidic cell separations.The Analyst · 2013Review
- Nanostructured substrates for isolation of circulating tumor cells.Nano today · 2013Article
- Stem Cell Separation Technologies.Current opinion in chemical engineering · 2013Article
- Generation of tissue constructs for cardiovascular regenerative medicine: from cell procurement to scaffold design.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Conventional cell separation against multiple markers generally requires the attachment of antibody tags, typically fluorescent or magnetic, to selected cell types in a heterogeneous suspension. This work describes how such separation can be accomplished in a series of microfluidic systems without the need for such tags. Two capture stages containing antibody-functionalized alginate hydrogels are utilized for the isolation of CD34+ and Flk1+ cells from untreated, whole human blood. The capture-release capability of these degradable coatings is harnessed by a mixing chamber and a simple valving system such that the suspension emerging from the first capture stage is prepared for the second capture stage for further enrichment. With this configuration, we demonstrate the isolation of CD34+/Flk1+ endothelial progenitor cells from blood enabled by the depletion of CD34+/Flk1-hematopoietic stem cells population. This ability to achieve isolation of cells against multiple markers in an untagged separation method is of particular significance in applications involving cell implantation-based therapeutics including tissue engineering and molecular analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.