ArticleAnalytical chemistry2012
Nanoflow low pressure high peak capacity single dimension LC-MS/MS platform for high-throughput, in-depth analysis of mammalian proteomes.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- High Dynamic Range Peptide Mass Spectrometry Using Segmented Precursor Ion Accumulation.Analytical chemistry · 2025Article
- Phosphoproteome Profiling of uEVs Reveals p-AQP2 and p-GSK3β as Potential Markers for Diabetic Nephropathy.Molecules (Basel, Switzerland) · 2023Article
- Optimized Sample Preparation and Microscale Separation Methods for High-Sensitivity Analysis of Hydrophilic Peptides.Molecules (Basel, Switzerland) · 2022Article
- Review
- Optofluidic flow meter for sub-nanoliter per minute flow measurements.Journal of biomedical optics · 2022Article
- Deep Profiling of Microgram-Scale Proteome by Tandem Mass Tag Mass Spectrometry.Journal of proteome research · 2021Article
- Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples.Analytical chemistry · 2019Article
- Nanowell-mediated multidimensional separations combining nanoLC with SLIM IM-MS for rapid, high-peak-capacity proteomic analyses.Analytical and bioanalytical chemistry · 2019Article
- Advances in microscale separations towards nanoproteomics applications.Journal of chromatography. A · 2017Review
- Differential contribution of the mitochondrial translation pathway to the survival of diffuse large B-cell lymphoma subsets.Cell death and differentiation · 2017Article
- Phosphoproteomic profiling of mouse primary HSPCs reveals new regulators of HSPC mobilization.Blood · 2016Article
- Comparison of commercial nanoliquid chromatography columns for fast, targeted mass spectrometry-based proteomics.Future science OA · 2016Article
- Systematic optimization of long gradient chromatography mass spectrometry for deep analysis of brain proteome.Journal of proteome research · 2015Article
- The Q Exactive HF, a Benchtop mass spectrometer with a pre-filter, high-performance quadrupole and an ultra-high-field Orbitrap analyzer.Molecular & cellular proteomics : MCP · 2014Article
- Picoelectrospray ionization mass spectrometry using narrow-bore chemically etched emitters.Journal of the American Society for Mass Spectrometry · 2014Article
- Long-gradient separations coupled with selected reaction monitoring for highly sensitive, large scale targeted protein quantification in a single analysis.Analytical chemistry · 2013Article
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
- Effects of column and gradient lengths on peak capacity and peptide identification in nanoflow LC-MS/MS of complex proteomic samples.Journal of the American Society for Mass Spectrometry · 2013Article
- Genome-scale proteome quantification by DEEP SEQ mass spectrometry.Nature communications · 2013Article
- Advanced proteomic liquid chromatography.Journal of chromatography. A · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The use of narrow bore LC capillaries operated at ultralow flow rates coupled with mass spectrometry provides a desirable convergence of figures of merit to support high-performance LC-MS/MS analysis. This configuration provides a viable means to achieve in-depth protein sequence coverage while maintaining a high rate of data production. Here we explore potential performance improvements afforded by use of 25 μm × 100 cm columns fabricated with 5 μm diameter reversed phase particles and integrated electrospray emitter tips. These columns achieve a separation peak capacity of ≈750 in a 600-min gradient, with average chromatographic peak widths of less than 1 min. At room temperature, a pressure drop of only ≈1500 psi is sufficient to maintain an effluent flow rate of ≤10 nL/min. Using mouse embryonic stem cells as a model for complex mammalian proteomes, we reproducibly identify over 4000 proteins across duplicate 600 min LC-MS/MS analyses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.