Evidence map›Paper›PMID 22518861›Full record

ArticleJournal of clinical microbiology2012

Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.

Milena Euler, Yongjie Wang, Peter Otto, Herbert Tomaso, Raquel Escudero, Pedro Anda, Frank T Hufert, Manfred Weidmann

Open access · bronzeAbstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed
5.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed, 156 citations in OpenAlex.

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12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Milena EulerUniversity Medical Center, Department of Virology, Göttingen, Germany.
Yongjie Wang
Peter Otto
Herbert Tomaso
Raquel Escudero
Pedro Anda
Frank T Hufert
Manfred Weidmann
University of Göttingen · DECentro Nacional de Microbiologia · ESFriedrich-Loeffler-Institut · DEShanghai Ocean University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.

Indexed as

AnimalsBacteriological TechniquesFrancisella tularensisHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPoint-of-Care SystemsSensitivity and SpecificityTemperatureTime FactorsTularemia

Identifiers

PMID22518861
PMCPMC3405570
OpenAlexW2097248933

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.