ArticleClinical and vaccine immunology : CVI2012
Identification and diagnostic utility of Leishmania infantum proteins found in urine samples from patients with visceral leishmaniasis.
Article in Clinical and vaccine immunology : CVI, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2020Pooled it
- Proteomic Analysis of Urine from Patients withTropical medicine and infectious disease · 2022Article
- Epidemiological and pathological characteristics of Cutaneous Leishmaniasis from Baluchistan Province of Pakistan.Parasitology · 2021Article
- Urine-Based Antigen (Protein) Detection Test for the Diagnosis of Visceral Leishmaniasis.Microorganisms · 2020Review
- Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods.Microorganisms · 2020Review
- Urine-based antigen detection assay for diagnosis of visceral leishmaniasis using monoclonal antibodies specific for six protein biomarkers of Leishmania infantum / Leishmania donovani.PLoS neglected tropical diseases · 2020Article
- Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients.PloS one · 2020Article
- Development of a Multiplexed Assay for Detection ofJournal of clinical microbiology · 2019Article
- Use of VHH antibodies for the development of antigen detection test for visceral leishmaniasis.Parasite immunology · 2018Article
- Development of a sandwich ELISA to detect Leishmania 40S ribosomal protein S12 antigen from blood samples of visceral leishmaniasis patients.BMC infectious diseases · 2018Article
- Discovery of a unique Mycobacterium tuberculosis protein through proteomic analysis of urine from patients with active tuberculosis.Microbes and infection · 2018Article
- Needle lost in the haystack: multiple reaction monitoring fails to detectF1000Research · 2018Article
- Novel Antigen Detection Assay to Monitor Therapeutic Efficacy of Visceral Leishmaniasis.The American journal of tropical medicine and hygiene · 2016Article
- Noninvasive Diagnosis of Visceral Leishmaniasis: Development and Evaluation of Two Urine-Based Immunoassays for Detection of Leishmania donovani Infection in India.PLoS neglected tropical diseases · 2016Article
- Development and comparative evaluation of two antigen detection tests for Visceral Leishmaniasis.BMC infectious diseases · 2015Article
- Potential non-invasive urine-based antigen (protein) detection assay to diagnose active visceral leishmaniasis.PLoS neglected tropical diseases · 2013Review
- Immunodiagnosis of Visceral Leishmaniasis: Current Status and Challenges: A Review Article.Iranian journal of parasitologyReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Despite the clear need to control visceral leishmaniasis (VL), the existing diagnostic tests have serious shortcomings. Here, we introduce an innovative approach to directly identify Leishmania infantum antigens produced in vivo in humans with VL. We combined reverse-phase high-performance liquid chromatography (RP-HPLC) with mass spectrometry and categorized three distinct L. infantum proteins presumably produced in bone marrow/spleen/liver and excreted in the urine of patients with VL. The genes coding for these proteins (L. infantum iron superoxide dismutase, NCBI accession number XP_001467866.1; L. infantum tryparedoxin, NCBI accession number XP_001466642.1; and L. infantum nuclear transport factor 2, NCBI accession number XP_001463738.1) were cloned, and the recombinant molecules were produced in Escherichia coli. Antibodies to these proteins were produced in rabbits and chickens and were used to develop a capture enzyme-linked immunosorbent assay (ELISA) designed to detect these L. infantum antigens in the urine of VL patients. Specificity of the antibodies was confirmed by a Western blot analysis using both recombinant proteins and whole parasite extract. Importantly, a urinary antigen detection assay assembled with pairs of antibodies specific for each of these antigens identified 17 of 19 patients with VL. These results indicate that an improved antigen detection assay based on L. infantum proteins present in the urine of patients with VL may represent an important new strategy for the development of a specific and accurate diagnostic test that has the potential to both distinguish active VL from asymptomatic infection and serve as an important tool to monitor therapy efficacy.
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Registered trials
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