ArticleNature protocols2012
A simple and rapid nonviral approach to efficiently transfect primary tissue-derived cells using polyethylenimine.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 111 citations in OpenAlex.
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- Polyethyleneimine-mediated gene transfection in porcine fetal fibroblasts.Animal reproduction · 2024Article
- Safe and Effective Delivery of mRNA Using Modified PEI-Based Lipopolymers.Pharmaceutics · 2023Article
- Nucleic acid direct delivery to fibroblasts: a review of nucleofection and applications.Journal of biological engineering · 2022Review
- Dihydroartemisinin protects blood-brain barrier permeability during sepsis by inhibiting the transcription factor SNAI1.Clinical and experimental pharmacology & physiology · 2022Article
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- Lanthanide-based β-Tricalcium Phosphate Upconversion Nanoparticles as an Effective Theranostic Nonviral Vectors for Image-Guided Gene Therapy.Nanotheranostics · 2022Article
- Establishing functional lentiviral vector production in a stirred bioreactor for CAR-T cell therapy.Bioengineered · 2021Article
- Design of transfections: Implementation of design of experiments for cell transfection fine tuning.Biotechnology and bioengineering · 2021Article
- Lymphatic PD-L1 Expression Restricts Tumor-Specific CD8Cancer research · 2021Article
- Strategies for High-Efficiency Mutation Using the CRISPR/Cas System.Frontiers in cell and developmental biology · 2021Review
- Article
- Comprehensive Flow Cytometry Analysis of PEI-Based Transfections for Virus-Like Particle Production.Research (Washington, D.C.) · 2020Article
- High-throughput screening of clinically approved drugs that prime nonviral gene delivery to human Mesenchymal stem cells.Journal of biological engineering · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This protocol outlines steps for optimizing the transfection of adherent primary mammalian cells using the readily available off-the-shelf cationic polymer, 25-kDa branched polyethylenimine (bPEI25). Transfection efficiency of cationic polymers varies among cell lines and is highly dependent on the conditions and environment in which complexes are formed. Factors requiring optimization include the salt concentration, volume, incubation time, mixing order and ratio of polymer to DNA. In this transfection protocol, complexes are prepared in 30 min, with analysis 24 h later; thus, experiments can be completed in 2 d. In this protocol, as an example, we describe the parameters we have optimized for the transfection of bone marrow stromal cells and normal human foreskin fibroblasts. By using this protocol, we have obtained transfection efficiencies comparable to lipofection. An appropriately optimized protocol enhances the utility of cationic polymers in transfecting mammalian cells, thereby providing an effective alternative to expensive commercial reagents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.